Alternate Names	T-cell surface glycoprotein CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Swissprot	P07766
References	1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Product Details

Form	Liquid
Clone No.	OKT-3(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802J]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5
	PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human CD3 Antibody[OKT-3]	50μg, 500μg, 1mg	Check More
Purified Anti-Human CD3 Antibody[OKT-3]	25μg, 100μg	Check More
Biotin Anti-Human CD3 Antibody[OKT-3]	25μg, 100μg	Check More
FITC Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD3 Antibody[OKT-3]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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