Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UJ
Cat.No.:E-AB-F1034UJ
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Alternate Names | P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162 |
Swissprot | Q62170 |
References |
1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942.
2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578. 3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422. |
Form | Liquid |
Clone No. | 4RA10(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG1, κ |
Isotype Control | PerCP/Cyanine5.5 Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823J] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PerCP/Cyanine5.5 |
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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