Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UJ

PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UJ

Uniprot : Q62170

Cat.No.:E-AB-F1034UJ
Applications: FCM
Isotype: Rat IgG1, κ
Host: Rat

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Antigen Details

Alternate Names	P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
Swissprot	Q62170
References	1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942. 2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578. 3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422.

Product Details

Form	Liquid
Clone No.	4RA10(See Other Available Formats)
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	PerCP/Cyanine5.5 Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823J]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5
	PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD162 Antibody[4RA10] E-AB-F10340	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034A	25μg, 100μg	Check More
Biotin Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034B	25μg, 100μg	Check More
FITC Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034E	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034J	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UD	25μg, 100μg	Check More
APC Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UE	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UG	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UI	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More

Citations

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