Alternate Names	Ly-1,Lyt-1,Lymphocyte antigen 1,CD5,Cd5
Swissprot	P13379
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.

Product Details

Form	Liquid
Clone No.	53-7.3(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833J]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5
	PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
FITC Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD5 Antibody[53-7.3]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
PE Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD5 Antibody[53-7.3]	25μg, 100μg	Check More

Citations

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