Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Mouse CD71 Antibody[R17 217.1.3/TIB-219]
Cat.No.:E-AB-F1093UJ
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Alternate Names | Transferrin receptor protein 1,Tfrc,TR,TfR,TfR1,Trfr,CD71 |
Swissprot | Q62351 |
References |
1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129. 3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403. 4. Kuhn LC, et al. 1984. Cell 37:95. |
Form | Liquid |
Clone No. | R17 217.1.3/TIB-219(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833J] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PerCP/Cyanine5.5 |
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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