Alternate Names	T-lymphocyte activation antigen CD80,Cd80,Activation B7-1 antigen,B7
Swissprot	Q00609
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Product Details

Form	Liquid
Clone No.	16-10A1(See Other Available Formats)
Host	Armenian Hamster
Isotype	Armenian Hamster IgG
Isotype Control	PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09853J]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5
	PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD80 Antibody[16-10A1]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
Biotin Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
FITC Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor 594 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor Violet 450 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor Red 780 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
PE Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
APC Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
PE/Elab Fluor 594 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
Elab Fluor Violet 450 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
Elab Fluor Red 780 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More

Citations

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