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Peroxidase (POD) Activity Assay Kit (Plant Samples)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K227-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $220
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(240 nm、420 nm)

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      Detection principle

      The peroxidase can catalyze the decomposition of H2O2 and produce water and oxygen. And oxygen oxidized pyrogallic acid to form yellow product. The activity of peroxidase can be calculated by measuring the absorbance at 420 nm.

      Performance characteristics

      Synonyms POD
      Sample type Plant tissue
      Sensitivity 0.5 U/mL
      Detection range 0.5-40 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 60 min
      Precision Average inter-assay CV: 5.5%Average intra-assay CV: 3.8%
      Other instruments required Incubator, Vortex mixer, Micropipettor, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months
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