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Pg(Progesterone) ELISA Kit

  • Cat.No.:E-EL-0154

  • Reactivity: Universal

To Purchase E-EL-0154

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.31-20 ng/mL
Sensitivity 0.15 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pg. During the reaction, Pg in the sample or standard competes with a fixed amount of Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.56 2.22 8.78 0.54 2.01 8.55
Standard deviation 0.05 0.14 0.53 0.05 0.14 0.53
CV (%) 8.39 6.42 5.98 9.35 7.04 6.21

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 80-94 84
EDTA plasma(n=8) 82-96 87
Urine(n=8) 95

Linearity

Samples were spiked with high concentrations of Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsProgesterone,Pg

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell Death & Disease (2017) IF: 5.965
    ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.

    DOI: 10.1038/cddis.2017.494

    PMID: 29072679

    Sample: Serum
  2. Journal of Biological Chemistry (2016) IF: 4.125
    Activation Of Pparγ And Cd36 Expression--The Dual Pathophysiological Roles Of Progesterone.

    DOI: 10.1074/jbc.M116.726737

    PMID: 27226602

    Sample: Serum
  3. Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606
    Protective roles and mechanisms of rosmarinic acid in cyclophosphamide-induced premature ovarian failure

    DOI: 10.1002/jbt.22591

    PMID: 32711407

    Sample: Serum
  4. International Journal of Molecular Medicine (2018) IF: 2.784
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    Sample: Serum
  5. Gene (2018) IF: 3.688
    Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1.

    DOI: 10.1016/j.gene.2018.05.072

    PMID: 29787826

    Sample: Cell culture medium
  6. Plos One (2017) IF: 2.806
    Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells.

    DOI: 10.1371/journal.pone.0188029

    PMID: 29125859

    Sample: Cell culture supernatant
  7. Reproduction in Domestic Animals (2018) IF: 1.4
    Methylation pattern variation between goats and rats during the onset of puberty.

    DOI: 10.1111/rda.13172

    PMID: 29577480

  8. Veterinary World (2020)
    Variability of serum reproductive hormones in cows presenting various reproductive conditions in semi-arid areas of the North West Province, South Africa

    DOI: 10.14202/vetworld.2020.502-507

    PMID: 32367956

    Sample: Serum
  9. AIP Conference Proceedings (2018)
    Combination effect of Centella asiatica (L.) urban and Pluchea indica (L.) urban on uterus weight and uterus and oviduct histological profiles of Rattus norwegicus

    DOI: 10.1063/1.5061907

    PMID: 99999999

    Sample: Serum
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Verified Customer

Z*ySubmitted [ Jun 01 2019 ]

  • Species:Human
  • Sample:serum
  • Sample source:serum
  • Detection result:good
  • Description:This ELISA Kit was used to assay normal human(women and men) serum by us. We operated procedure as Elabscience,s the instruction manual provided and the experimental results were very good------when the human serums were detected without dilution, the testing results were in the range of standard curve, what’s more, It is very near compared with the clinical assay kit! The Pg level of normal men serum is 0.1-3.5 ng/mL and the follicular phase women serum is 0.1-2.1 ng/mL.
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