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Pg(Progesterone) ELISA Kit

Name: Pg(Progesterone) ELISA Kit
Brand: Elabscience
SKU: E-EL-0154
Price: 495 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(9)

Cat.No.:E-EL-0154
Reactivity: Universal

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	0.31-20 ng/mL
Sensitivity	0.15 ng/mL
Sample type &Sample volume	serum, plasma, urine, saliva; 50μL
Specificity	This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pg. During the reaction, Pg in the sample or standard competes with a fixed amount of Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.56	2.22	8.78	0.54	2.01	8.55
Standard deviation	0.05	0.14	0.53	0.05	0.14	0.53
CV (%)	8.39	6.42	5.98	9.35	7.04	6.21

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	80-94	84
EDTA plasma(n=8)	82-96	87
Urine(n=8)	85-109	95

Linearity

Samples were spiked with high concentrations of Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Signal Transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Cell Death & Disease (2017) IF: 5.965

ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.

DOI: 10.1038/cddis.2017.494
PMID: 29072679	Sample: Serum

Journal of Biological Chemistry (2016) IF: 4.125

Activation Of Pparγ And Cd36 Expression--The Dual Pathophysiological Roles Of Progesterone.

DOI: 10.1074/jbc.M116.726737
PMID: 27226602	Sample: Serum

Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606

Protective roles and mechanisms of rosmarinic acid in cyclophosphamide-induced premature ovarian failure

DOI: 10.1002/jbt.22591
PMID: 32711407	Sample: Serum

International Journal of Molecular Medicine (2018) IF: 2.784

Expression of FKBP52 in the ovaries of PCOS rats

DOI: 10.3892/ijmm.2018.3998
PMID: 30483787	Sample: Serum

Gene (2018) IF: 3.688

Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1.

DOI: 10.1016/j.gene.2018.05.072
PMID: 29787826	Sample: Cell culture medium

Plos One (2017) IF: 2.806

Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells.

DOI: 10.1371/journal.pone.0188029
PMID: 29125859	Sample: Cell culture supernatant

Reproduction in Domestic Animals (2018) IF: 1.4

Methylation pattern variation between goats and rats during the onset of puberty.

DOI: 10.1111/rda.13172
PMID: 29577480

Veterinary World (2020)

Variability of serum reproductive hormones in cows presenting various reproductive conditions in semi-arid areas of the North West Province, South Africa

DOI: 10.14202/vetworld.2020.502-507
PMID: 32367956	Sample: Serum

AIP Conference Proceedings (2018)

Combination effect of Centella asiatica (L.) urban and Pluchea indica (L.) urban on uterus weight and uterus and oviduct histological profiles of Rattus norwegicus

DOI: 10.1063/1.5061907
	Sample: Serum

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Reviews/Q&A

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Q&A

Verified Customer

Z*ySubmitted [ Jun 01 2019 ]

Species:Human
Sample:serum
Sample source:serum
Detection result:good
Description:This ELISA Kit was used to assay normal human(women and men) serum by us. We operated procedure as Elabscience,s the instruction manual provided and the experimental results were very good------when the human serums were detected without dilution, the testing results were in the range of standard curve, what’s more, It is very near compared with the clinical assay kit! The Pg level of normal men serum is 0.1-3.5 ng/mL and the follicular phase women serum is 0.1-2.1 ng/mL.

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A*kSubmitted [ Feb 04 2023 ]

Asked: Is it use for bovine? This kit applicable or not?

adminSubmitted [ Feb 04 2023 ]

Answered: This kit can be used for bovine samples, we have validated the kit with normal bovine serum/plasma samples, the reference values were about 0.49-3.97ng/mL.

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