×

PGE2(Prostaglandin E2) ELISA Kit

  • Cat.No.:E-EL-0034

  • Reactivity: Universal

To Purchase E-EL-0034

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes PGE2 in samples. No significant cross-reactivity or interference between PGE2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE2. During the reaction, PGE2 in the sample or standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGE2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGE2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 101.30 236.80 779.30 96.00 259.90 728.60
Standard deviation 6.60 11.60 42.10 4.90 13.80 31.30
CV (%) 6.52 4.90 5.40 5.10 5.31 4.30

Recovery

The recovery of PGE2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-109 99
EDTA plasma(n=8) 87-99 94
Cell culture media(n=8) 86-96 91

Linearity

Samples were spiked with high concentrations of PGE2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsPG-E2, Dinoprostone

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal for immunotherapy of cancer (2020) IF: 9.913
    Melanoma-specific bcl-2 promotes a protumoral M2-like phenotype by tumor-associated macrophages

    DOI: 10.1136/jitc-2019-000489

    PMID: 32269145

    Sample: Cell sample
  2. development (2019) IF: 5.413
    Effects of Tarantula cubensis alcoholic extract and Nerium oleander distillate on experimentally induced colon cancer

    PMID: 99999999

    Sample: Serum
  3. Journal of Crohn's & Colitis (2015) IF: 5.813
    Diet Induced Hyperhomocysteinemia Increases Intestinal Inflammation In An Animal Model Of Colitis.

    DOI: 10.1093/ecco-jcc/jjv094

    PMID: 26071411

    Sample: Plasma
  4. Journal of Reproductive Immunology (2020) IF: 4.018
    Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling

    DOI: 10.1016/j.jri.2020.103192

    PMID: 32950783

    Sample: Cell culture supernatant
  5. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling

    DOI: 10.1021/acs.jafc.0c03123

    PMID: 32797754

    Sample: Cell culture medium
  6. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    Sample: Serum,Tissue homogenate
  7. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction

    DOI: 10.1016/j.biopha.2020.109826

    PMID: 31978766

    Sample: Tissue homogenate
  8. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Huoxuezhitong capsule ameliorates MIA-induced osteoarthritis of rats through suppressing PI3K/ Akt/ NF-κB pathway

    DOI: 10.1016/j.biopha.2020.110471

    PMID: 99999999

    Sample: Serum
  9. Frontiers in Pharmacology (2019) IF: 4.225
    The Role of Prostaglandin-Endoperoxide Synthase-2 in Chemoresistance of Non-Small Cell Lung Cancer

    DOI: 10.3389/fphar.2019.00836

    PMID: 31440159

    Sample: Cell culture supernatant
  10. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice

    DOI: 10.1016/j.ecoenv.2020.110698

    PMID: 32388187

    Sample: Cell culture supernatant
  11. Science of The Total Environment (2019) IF: 4.61
    Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

    DOI: 10.1016/j.scitotenv.2019.02.419

    PMID: 30833257

    Sample: Plasma
  12. Inflammopharmacology (2020) IF: 3.238
    Moringa rivae leaf extracts attenuate Complete Freund's adjuvant-induced arthritis in Wistar rats via modulation of inflammatory and oxidative stress biomarkers

    DOI: 10.1007/s10787-019-00596-3

    PMID: 31037575

    Sample: Serum
  13. The International Journal of Biochemistry & Cell Biology (2019) IF: 3.673
    Cisplatin induces chemoresistance through the PTGS2-mediated anti-apoptosis in gastric cancer

    DOI: 10.1016/j.biocel.2019.105610

    PMID: 31518663

    Sample: Cell culture supernatant
  14. Phytomedicine (2018) IF: 3.61
    Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways

    DOI: 10.1016/j.phymed.2018.11.015

    PMID: 31005814

    Sample: Cell culture supernatant
  15. Plants (2020) IF: 2.762
    Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

    DOI: 10.3390/plants9091127

    PMID: 32878164

    Sample: Cell culture supernatant
  16. Pharmacological Reports (2020) IF: 2.754
    Differential effects of performance-enhancing drugs 'Methamphetamine' and 'hCG' on ex-vivo cultured primary blood mononuclear cells of male athletes

    DOI: 10.1007/s43440-020-00066-6

    PMID: 32124391

    Sample: Cell culture supernatant
  17. PloS one (2019) IF: 2.74
    LL-37 alone and in combination with IL17A enhances proinflammatory cytokine expression in parallel with hyaluronan metabolism in human synovial sarcoma cell line SW982—A step toward understanding the development of inflammatory arthritis

    DOI: 10.1371/journal.pone.0218736

    PMID: 31260471

    Sample: Cell culture supernatant
  18. Steroids (2019) IF: 2.523
    Synthesis and Evaluation of Novel D-ring Substituted Steroidal Pyrazolines as Potential Anti-inflammatory Agents

    DOI: 10.1016/j.steroids.2019.03.012

    PMID: 30951758

    Sample: Cell culture supernatant
  19. Biological trace element research (2018) IF: 2.361
    Melatonin Ameliorates Neuropharmacological and Neurobiochemical Alterations Induced by Subchronic Exposure to Arsenic in Wistar Rats

    DOI: 10.1007/s12011-018-1537-1

    PMID: 30306420

    Sample: Tissue homogenate
  20. BioMed Research International (2019) IF: 2.197
    Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    DOI: 10.1155/2019/3018357

    PMID: 31240210

    Sample: Cell culture supernatant
  21. Journal of Pain Research (2017) IF: 2.581
    Involvement Of Endoplasmic Reticulum Stress In Formalin-Induced Pain Is Attenuated By 4-Phenylbutyric Acid.

    DOI: 10.2147/JPR.S125805

    PMID: 28360534

    Sample: Cerebrospinal fluid
  22. Journal of Otolaryngology-Head & Neck Surgery (2020) IF: 1.852
    Simple hypertrophic tonsils have more active innate immune and inflammatory responses than hypertrophic tonsils with recurrent inflammation in children

    DOI: 10.1186/s40463-020-00428-3

    PMID: 32487224

    Sample: Tissue homogenate
  23. Medicine (2019) IF: 1.552
    Role of ascitic prostaglandin E2 in diagnosis of spontaneous bacterial peritonitis and prediction of in-hospital mortality in patients with decompensated cirrhosis

    DOI: 10.1097/MD.0000000000016016

    PMID: 31261505

    Sample: Ascitic fluid
  24. Neuroreport (2018) IF: 1.394
    Gentiopicroside protects neurons from astrocyte-mediated inflammatory injuries by inhibition of nuclear factor-κB and mitogen-activated protein kinase signaling pathways

    DOI: 10.1097/WNR.0000000000001082

    PMID: 29958245

    Sample: Cell culture supernatant
  25. Experimental animals (2018) IF: 1.173
    Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats.

    DOI: 10.1538/expanim.18-0029

    PMID: 29731490

    Sample: Serum
  26. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research (2017) IF: 1.585
    Cross-Coupling Effects Of Silencing Of Cyclooxygenase-2 (COX-2)/Aggrecanase-1 And Over-Expressed Insulin-Like Growth Factor 1 (IGF-1) In An Osteoarthritis Animal Model.

    DOI: 10.12659/MSM.907150

    PMID: 29112682

    Sample: synovial fluid
  27. Journal of Pharmaceutical Sciences and Research (2017)
    Study of some Biochemical parameter in patients infected with Giardia lamblia

    PMID: 99999999

    Sample: Serum
  28. Journal of Clinical & Experimental Investigations (2019)
    Age-dependent Role of Cilostazol on Cold Restraint Stressinduced Gastric Ulceration in Female Rats

    DOI: 10.5799/jcei/5902

    PMID: 99999999

    Sample: Tissue homogenate
  29. Preprint (2020)
    Protective effects of Schisandrin C on chondrocyte damage by inhibiting MAPK and NF-κB signal pathways

    DOI: 10.21203/rs.3.rs-32659/v1

    PMID: 99999999

    Sample: Cell culture supernatant
  30. Journal of BU ON.: official journal of the Balkan Union of Oncology (2019)
    Effects of postoperative enteral nutrition combined with adjuvant radiotherapy on inflammatory response, nutrition, healing and prognosis in patients receiving radical surgery for esophageal carcinoma

    PMID: 31646824

    Sample: Serum
  31. The American Journal of Chinese Medicine (2020)
    Corydalis saxicola Alkaloids Attenuate Cisplatin-Induced Neuropathic Pain by Reducing Loss of IENF and Blocking TRPV1 Activation

    DOI: 10.1142/S0192415X20500214

    PMID: 32138533

    Sample: Serum
  32. Pathophysiology (2019)
    Pathophysiology of iron overload-induced renal injury and dysfunction: Roles of renal oxidative stress and systemic inflammatory mediators

    DOI: 10.1016/j.pathophys.2019.03.002

    PMID: 30910397

    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?

*Captcha: