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Cat.No.:E-EL-0034
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes PGE2 in samples. No significant cross-reactivity or interference between PGE2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGE2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGE2 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 101.30 | 236.80 | 779.30 | 96.00 | 259.90 | 728.60 |
Standard deviation | 6.60 | 11.60 | 42.10 | 4.90 | 13.80 | 31.30 |
CV (%) | 6.52 | 4.90 | 5.40 | 5.10 | 5.31 | 4.30 |
The recovery of PGE2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-109 | 99 |
EDTA plasma(n=8) | 87-99 | 94 |
Cell culture media(n=8) | 86-96 | 91 |
Samples were spiked with high concentrations of PGE2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | PG-E2, Dinoprostone |
Research Area | Signal Transduction |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
PMID: 36351432 |
Sample: MDSCs,B16 cell,MC38 cell |
PMID: 32269145 |
Sample: Cell sample |
Sample: plasma |
PMID: 36184177 |
Sample: bronchoalveolar fluid,RAW264.7 cell |
Sample: Plasma |
DOI: 10.7554/eLife.54523 |
|
Sample: MSC |
PMID: 33465467 |
Sample: Tissue homogenate(colon and uterus) |
PMID: 36122518 |
Sample: gastric mucosal tissue, |
PMID: 33828553 |
Sample: Cell lysate |
DOI: 10.1039/D2TB01389D |
|
PMID: 34015638 |
Sample: Serum |
PMID: 32388187 |
Sample: Cell culture supernatant |
PMID: 34537445 |
Sample: brain tissue |
PMID: 33711550 |
Sample: Whole Blood |
PMID: 32179202 |
Sample: Tissue homogenate |
PMID: 31978766 |
Sample: Tissue homogenate |
DOI: 10.3390/cells8070739 |
|
Sample: PC12 cells,BV2 cells |
Sample: serum |
DOI: 10.2147/JIR.S317182 |
|
Sample: U937 cell |
PMID: 33193319 |
Sample: Plasma |
Sample: prostatic tissue |
PMID: 36280015 |
Sample: RAW264.7 cells |
Sample: serum,RAW 264.7 cells |
DOI: 10.3390/ph15091097 |
|
PMID: 36145318 |
Sample: HT-29 cell |
PMID: 34735907 |
Sample: colon tissue |
Sample: gastric mucosa |
PMID: 34582971 |
Sample: serum |
Sample: BV2 cell,C6 cell |
PMID: 30833257 |
Sample: Plasma |
Sample: serum |
PMID: 36059979 |
Sample: stomach tissue |
Sample: stomach tissue |
Sample: Stomach tissue |
Sample: MH7A cell |
PMID: 32950783 |
Sample: Cell culture supernatant |
Sample: RAW264.7 cells |
PMID: 32797754 |
Sample: Cell culture medium |
PMID: 34530094 |
Sample: serum |
DOI: 10.1111/cas.13497 |
|
Sample: serum |
PMID: 34045549 |
Sample: Tissue homogenate |
PMID: 35458665 |
Sample: serum |
PMID: 35408620 |
Sample: Liver tissues |
Sample: Cell culture supernatant |
Sample: BV2 cells |
Sample: larvae |
PMID: 34106384 |
Sample: serum |
Sample: serum |
Sample: serum |
PMID: 35022916 |
Sample: Colon adenocarcinoma (HT-29) cell |
Sample: Serum |
PMID: 33827384 |
Sample: peyer’s patches (PPs),RAW 264.7 cell |
PMID: 36289770 |
Sample: liver tissue |
Sample: Primary TGNs |
PMID: 36144661 |
Sample: stomach tissue |
Sample: hippocampa |
PMID: 35807509 |
Sample: human fibroblast cell |
PMID: 35736412 |
Sample: cerebrospinal fluid (CSF) |
PMID: 35259712 |
Sample: blood |
PMID: 35366641 |
Sample: colon tissues |
PMID: 34009457 |
Sample: Serum |
Sample: peritoneal macrophages (PMs) |
DOI: 10.1530/REP-19-0196 |
|
Sample: ESCs cells |
PMID: 31518663 |
Sample: Cell culture supernatant |
Sample: brain |
PMID: 33957357 |
Sample: Tissue homogenate(synovial) |
Sample: serum,ADTC5 cell |
Sample: serum |
DOI: 10.1039/C9FO01342C |
|
Sample: serum |
DOI: 10.1002/nau.23470 |
|
Sample: serum |
Sample: serum |
Sample: serum |
DOI: 10.3390/pr10101985 |
|
Sample: gastric tissue |
DOI: 10.3390/pr10071314 |
|
Sample: stomach |
Sample: BV-2 cells |
DOI: 10.1155/2022/4117520 |
|
PMID: 35509713 |
Sample: plasma |
PMID: 33705895 |
Sample: Cell culture supernatant |
Sample: Plasma |
PMID: 31005814 |
Sample: Cell culture supernatant |
Sample: macrophages |
DOI: 10.1002/jbt.22932 |
|
PMID: 34665902 |
Sample: serum,intestianl tissue |
PMID: 32138533 |
Sample: Serum |
PMID: 34339004 |
Sample: aorta |
Sample: Cell culture medium,Tissue Slice |
Sample: Serum |
PMID: 31440159 |
Sample: Cell culture supernatant |
DOI: 10.21037/atm-21-684 |
|
PMID: 34430561 |
Sample: serum |
Sample: CHME-5 cells,RRID cells,CVCL_5J53 cells,U87MGcells |
PMID: 33047165 |
Sample: Serum |
PMID: 30951758 |
Sample: Cell culture supernatant |
Sample: paw tissue |
DOI: 10.1155/2019/3018357 |
|
PMID: 31240210 |
Sample: Cell culture supernatant |
PMID: 33383182 |
Sample: biological fluids |
PMID: 30306420 |
Sample: Tissue homogenate |
Sample: MLO-Y4 cell |
Sample: SW1353 cell |
Sample: HRPE cell |
DOI: 10.2147/JPR.S125805 |
|
Sample: Cerebrospinal fluid |
PMID: 35906691 |
Sample: Stomach tissue |
DOI: 10.1155/2021/9958808 |
|
Sample: THP-1 cells |
DOI: 10.1155/2021/6613140 |
|
Sample: Tissue homogenate |
DOI: 10.1155/2022/1695701 |
|
PMID: 35422870 |
Sample: serum |
DOI: 10.1155/2022/7008907 |
|
PMID: 35873638 |
Sample: RAW264.7 cell |
PMID: 34978233 |
Sample: stomach tissue |
Sample: stomach tissue |
PMID: 33836205 |
Sample: Cell culture supernatant |
Sample: RAW 264.7 cell |
DOI: 10.1111/jfbc.13859 |
|
PMID: 34258791 |
Sample: serum |
Sample: serum |
Sample: Cell culture supernatant |
PMID: 32878164 |
Sample: Cell culture supernatant |
PMID: 35148687 |
Sample: serum |
Sample: AGS cell |
PMID: 34523697 |
Sample: RAW264.7 cell |
Sample: LO2 cell |
Sample: Serum |
Sample: RAW 264.7 cells |
PMID: 29958245 |
Sample: Cell culture supernatant |
PMID: 31646824 |
Sample: serum |
DOI: 10.12659/MSM.907150 |
|
Sample: synovial fluid |
DOI: 10.1111/jfbc.13258 |
|
Sample: serum |
DOI: 10.1155/2020/8836080 |
|
PMID: 33381210 |
Sample: Serum |
Sample: serum |
PMID: 32487224 |
Sample: Tissue homogenate |
PMID: 31261505 |
Sample: Ascitic fluid |
PMID: 33181144 |
Sample: Tissue homogenate |
Sample: serum |
Sample: plasma |
Sample: RAW264.7 cell |
Sample: SW480 cell |
L**************nSubmitted [ Mar 08 2022 ]
Asked: Hi there! I need PGE2 elisa kit that capable to detect PGE2 from RAW and Caco2 cell. is your product (E-EL-0034) capable to detect from mouse and human cell line? Hope to see your response soon. Thank u
Reply
adminSubmitted [ Mar 08 2022 ]
Answered: Dear Setiawan, GREETINGS FROM ELABSCIENCE! E-EL-0034 PGE2(Prostaglandin E2) ELISA Kit It can bes used to detect PGE2 from RAW and Caco2 from mouse and human cell line. Any questions pls feel free to ask ya~ It's my email address: Amanda@elabscience.com