PHF21A Polyclonal Antibody
Price: $ 530
Price: $ 320
Price: $ 200
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:U87-MG,293T,Mouse brain,Rat brain |
Dilution |
WB 1:500-1:2000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human PHF21A (NP_001095272.1). |
Abbre | PHF21A |
Synonyms | PHF21A;BHC80;BM-006 |
Swissprot | |
Calculated MW | 70kDa/74kDa |
Observed MW |
70-80kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Epigenetics and Nuclear Signaling; Neuroscience |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. PHF21A (PHD finger protein 21A), also known as BRAF35-HDAC complex protein BHC80, is a 680 amino acid nuclear protein that contains one PHD-type zinc finger and one A.T hook DNA-binding domain, suggesting involvement in transcriptional regulation events. PHF21A is a component of the BHC complex, which is responsible for repressing transcription of neuron-specific genes in non-neuronal cells. The BHC complex acts as a chromatin modifier that deacetylates and demethylates specific sites on histones. PHF21A may act as a scaffold within the BHC complex. Predominantly expressed in brain, three isoforms of PHF21A exist as a result of alternative splicing events. |