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Phospho-CREB1 (Ser133) Polyclonal Antibody

Cat:E-AB-20849 Citations (1)
Manual MSDS
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Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
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Product Details
Verified Samples Verified Samples in WB:MCF-7,A549,K562,HEK293
Verified Samples in IHC:Human uterus
Verified Samples in IF:Mouse lung
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from human CREB-1 around the phosphorylation site of Ser133
Abbre CREB-1 (phospho Ser133)
Synonyms Active transcription factor CREB;cAMP response element binding protein 1;cAMP response element binding protein;cAMP responsive element binding protein 1;cAMP-responsive element-binding protein 1;CREB;CREB-1;CREB1;CREB1;Cyclic AMP-responsive element-binding protein 1;MGC9284;OTTHUMP00000163864;OTTHUMP00000163865;OTTHUMP00000206660;OTTHUMP00000206662;OTTHUMP00000206667;Transactivator protein
Swissprot
Calculated MW 37kDa
Observed MW 37kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Immunology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.