Phospho-CREB1 (Ser133) Polyclonal Antibody
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Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC-p;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:MCF-7,A549,K562,HEK293 Verified Samples in IHC:Human uterus Verified Samples in IF:Mouse lung |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Synthesized peptide derived from human CREB-1 around the phosphorylation site of Ser133 |
Abbre | CREB-1 (phospho Ser133) |
Synonyms | Active transcription factor CREB;cAMP response element binding protein 1;cAMP response element binding protein;cAMP responsive element binding protein 1;cAMP-responsive element-binding protein 1;CREB;CREB-1;CREB1;CREB1;Cyclic AMP-responsive element-binding protein 1;MGC9284;OTTHUMP00000163864;OTTHUMP00000163865;OTTHUMP00000206660;OTTHUMP00000206662;OTTHUMP00000206667;Transactivator protein |
Swissprot | |
Calculated MW | 37kDa |
Observed MW |
37kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling; Immunology |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. |
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Oleuropein confers neuroprotection against rotenone-induced model of Parkinson’s disease via BDNF/CREB/Akt pathway
IF:4.996
Journal:Scientific Reports