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Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)

Cat.No.:E-BC-K245-M
Detection instrument: Microplate reader(620 nm-690 nm,optimum wavelength: 660 nm)

Detection method: Colorimetric method

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Detection principle

Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.

Performance characteristics

Synonyms	Pi
Sample type	Serum,plasma,tissue
Sensitivity	0.004 mmol/L
Detection range	0.004-2.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 3%Average intra-assay CV: 2.100%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.004-2.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Rat plasma	1
Human urine	2-3
10% Rat liver tissue homogenate	1
10% Mouse lung tissue homogenate	1
10% Mouse brain tissue homogenate	1
10% Rat muscle tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

International Journal of Nanomedicine (2022) IF: 6.4
A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

DOI: 10.2147/IJN.S351814

PMID: 35299865
Sample: BPN-DFO hydrogel
Frontiers in Physiology (2023) IF: 4.755
Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725

Sample: Serum

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