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Plant Flavonoids Colorimetric Assay Kit

Cat.No.:E-BC-K284-M
Detection instrument: Microplate reader(500 nm-520 nm,optimum wavelength: 510 nm)

Detection method: Colorimetric method

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Detection principle

In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorbance of the sample extract at 510 nm.

Performance characteristics

Sample type	Plant tissue
Sensitivity	0.66 μg/mL
Detection range	0.66-150 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	35 min
Precision	Average inter-assay CV: 5.300%Average intra-assay CV: 4%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Vacuum dryer, Ultrasonic cell disruptor.
Other reagents required	60% alcohol
Storage	2-8℃
Valid period	12 months

Images

The concentration of total flavonoid was determined.

JH Kim et al investigate the bisphenol A-induced inflammation and cytotoxicity blocked by Grifola frondosa extract. Plant flavonoids level in plant tissue was determined using plant flavonoids colorimetric assay kit (E-BC-K284-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.66-150 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Green dill	10-15
Green pepper	1
Pumpkin	1
Heather	25-35

Note: The diluent is 60% alcohol.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nutrients (2022) IF: 6.706
Grifola frondosa Extract Containing Bioactive Components Blocks Skin Fibroblastic Inflammation and Cytotoxicity Caused by Endocrine Disrupting Chemical, Bisphenol A

DOI: 10.3390/nu14183812

PMID: 36145189
Sample: fruit

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