Plant Flavonoids Colorimetric Assay Kit (E-BC-K284-M)
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For research use only.
| Detection Principle | In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorbance of the sample extract at 510 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | 60% Absolute ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.66 μg/mL |
| Detection Range | 0.66-150 μg/mL |
| Precision | inter-assay CV: 5.3% | intra-assay CV: 4% |
| Sample Volume | 75 μL(tissue homogenate) |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Epipremnum aureum | 10-15 |
| Green pepper | 1 |
| Pumpkin | 1 |
| Heather | 25-35 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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