Plant Nitrate Nitrogen Colorimetric Assay Kit (E-BC-K858-M)

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All	Size	Price	Qty
	96T	$ 320.00
	48T	$ 240.00

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Detection Principle	Nitrate nitrogen can react with salicylic acid to produce nitrosalicylic acid in a strongly acidic environment. Nitrosalicylic acid is yellow under strong alkaline conditions, and the depth of color is proportional to the content at a wavelength of 410 nm.
Sample Type	plant tissue,Soil
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (405-415 nm, optimum wavelength: 410 nm)
Research Area	Plant Physiology Series
Other Reagents Required	Potassium nitrate, Concentrated sulfuric acid
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.09 μg/mL
Detection Range	1.09-200 μg/mL
Precision	inter-assay CV: 3.2-5.1% \| intra-assay CV: 1.5-4.3%
Sample Volume	10 μL(tissue homogenate)
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K177-M	Proline (Pro) Colorimetric Assay Kit	96T , 48T
E-BC-K860-M	Proline Dehydrogenase (ProDH) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
20% Epipremnum aureum root tissue homogenization	1
20% Soil homogenization	1
20% Core leaves tissue homogenization	1
20% Green pepper tissue homogenization	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.