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Plant Root Activity Assay Kit

Cat.No.:E-BC-K855-S
Detection instrument: Spectrophotometer (485 nm)

Detection method: Colorimetric method

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100 Assays

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★: Microplate is not provided in metabolism assay kits of over 500 assays.

Detection principle

The detection principle of the kit is to use 2，3，5-triphenyltetrazolium chloride (TTC) as the substrate, incubate for 1 to 3 hours, the dehydrogenase in the root can reduce TTC and generate 1,3,5-tiphenylformazan (TTF) which is insoluble in water, then TTF will be extracted from the root with organic solvent (ethyl acetate or acetone, etc.) By detecting the absorbance at 485nm, the amount of TTC reduction can be calculated, which represents the dehydrogenase activity and is used as an index of plant root activity. The kit is used for quantitative determination of plant root activity or dehydrogenase activity.

Performance characteristics

Sample type	plant tissue
Sensitivity	0 U/g wet weight
Detection range	0-0.67 U/g wet weight
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	3.5h
Precision	Average inter-assay CV: 3%Average intra-assay CV: 1%
Other instruments required	Centrifuge, Incubator
Other reagents required	Double distilled water, Ethyl Acetate
Storage	-20℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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