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Plant Root Activity Assay Kit

  • Cat.No.:E-BC-K855-S

  • Detection instrument: Spectrophotometer (485 nm)

To Purchase E-BC-K855-S

  • 100 Assays
Price: $220

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Protocols for metabolism assays

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★: Microplate is not provided in metabolism assay kits of over 500 assays.

Detection principle

The detection principle of the kit is to use 2,3,5-triphenyltetrazolium chloride (TTC) as the substrate, incubate for 1 to 3 hours, the dehydrogenase in the root can reduce TTC and generate 1,3,5-tiphenylformazan (TTF) which is insoluble in water, then TTF will be extracted from the root with organic solvent (ethyl acetate or acetone, etc.) By detecting the absorbance at 485nm, the amount of TTC reduction can be calculated, which represents the dehydrogenase activity and is used as an index of plant root activity. The kit is used for quantitative determination of plant root activity or dehydrogenase activity.

Performance characteristics

Sample type plant tissue
Sensitivity 0 U/g wet weight
Detection range 0-0.67 U/g wet weight
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 3.5h
Precision Average inter-assay CV: 3%Average intra-assay CV: 1%
Other instruments required Centrifuge, Incubator
Other reagents required Double distilled water, Ethyl Acetate
Storage -20℃
Valid period 12 months


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