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PLEKHF2 Polyclonal Antibody

Cat:E-AB-19192
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:Mouse kidney,Raji,Jurkat
Verified Samples in IHC:Human tonsil
Dilution

WB 1:1000-1:5000, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Fusion protein of human PLEKHF2
Abbre PLEKHF2
Synonyms 110070J07Rik;AA673237;FLJ13187;PH and FYVE domain containing protein 2;PH and FYVE domain-containing protein 2;PH domain containing family F member 2;PH domain-containing family F member 2;Phafin 2;Phafin-2;PHAFIN2;PKHF2;pleckstrin homology domain containing; family F (with FYVE domain) member 2;pleckstrin homology domain containing; family F (with FYVE domain) member2 ;Pleckstrin homology domain-containing family F member 2;Plekhf2;ZFYVE18;Zinc finger FYVE domain containing protein 18 ;Zinc finger FYVE domain-containing protein 18
Swissprot
Calculated MW 28 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1.62 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cell Biology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis (PubMed:18288467).