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POMC Polyclonal Antibody

Cat:E-AB-40635
Manual MSDS

Price: $ 230

Price: $ 125

Size:
100μL 50μL
Quantity:
  • Host: Rabbit
  • Reactivity: Mouse;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Mouse pituitary,Rat pituitary
Dilution

WB 1:1000-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide.
Abbre POMC
Synonyms Pro-opiomelanocortin;Corticotropin-lipotropin;NPP;Melanotropin gamma;Gamma-MSH;Potential peptide;Corticotropin;ACTH;CLIP;Alpha-MSH;Beta-LPH;Gamma-LPH;Beta-MSH;Beta-endorphin;Met-enkephalin
Swissprot
Calculated MW 29 kDa
Observed MW 35 kDa, 16 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted
Concentration 1.8mg/mL
Buffer PBS with 0.02% sodium azide,50% glycerol,pH 7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer; Metabolism; Neuroscience; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Pro-opiomelanocortin (POMC) is a precursor protein expressed in the pituitary and the brain where it is processed into several peptide hormones and neuropeptides. Among these peptides are ACTH,α- and β-MSH,β-and γ-LPH,CLIP,β-endorphin,and N-POMC. POMC is involved in hypothalamic circuits regulating feeding behavior and POMC-producing neurons promote satiety. POMC neurons are also the target of leptin and insulin for the promotion of the browning of white fat.