English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

PRDX4 Polyclonal Antibody

Cat:E-AB-19430
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB;IHC
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:293T,Raji,Hela
Verified Samples in IHC:Human cervical cancer
Dilution

WB 1:500-1:2000, IHC 1:25-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human PRDX4
Abbre PRDX4
Synonyms Antioxidant enzyme 372;Antioxidant enzyme AOE372;AOE37 2;AOE37-2;AOE372;EC 1.11.1.15;Peroxiredoxin IV;Peroxiredoxin-4;Peroxiredoxin4;PRDX 4;Prdx4;PRDX4;PRX 4;Prx IV;Prx-IV;PRX4;PrxIV;Thioredoxin dependent peroxide reductase A0372;Thioredoxin Peroxidase (Antioxidant Enzyme);Thioredoxin peroxidase;Thioredoxin peroxidase AO372;Thioredoxin-dependent peroxide reductase A0372;TRANK
Swissprot
Calculated MW 30 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Secreted.
Concentration 0.8 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Cell Biology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background PRDX4(Peroxiredoxin-4) is also named as AOE37-2,Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract,because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage. It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies. PRDX4 is a pentamer of dimers.