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PRKCSH Polyclonal Antibody

Cat:E-AB-53187
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:HepG2
Verified Samples in IHC:Human colorectal cancer,Human brain
Dilution

WB 1:500-1:2000, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Fusion protein of human PRKCSH
Abbre PRKCSH
Synonyms 80K-H protein;AGE-binding receptor 2;AGE-R2;G19P1;GLU2B;Glucosidase 2 subunit beta;Glucosidase II beta subunit;Glucosidase II subunit beta;Hepatocystin;PCLD;PKCSH;PLD1;PRKCSH;Protein kinase C substrate 60.1 kDa protein heavy chain;Protein kinase C substrate 80 Kda protein;Protein kinase C substrate 80K-H;Protein kinase C substrate;80 Kda protein
Swissprot
Calculated MW 59 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Endoplasmic reticulum.
Concentration 1.32 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Signal Transduction; Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.PRKCSH (Protein Kinase C Substrate 80K-H) is a Protein Coding gene. Diseases associated with PRKCSH include Polycystic Liver Disease and Polycystic Kidney And Hepatic Disease. Among its related pathways are Advanced glycosylation endproduct receptor signaling and Innate Immune System. GO annotations related to this gene include calcium ion binding and ion channel binding.