Proline Dehydrogenase (ProDH) Activity Assay Kit (E-BC-K860-M)
For research use only.
| Detection Principle | The hydrogen produced by proline during the catalytic process of ProDH can reduce the chromogenic agent through electron acceptor, and lighten the color. The ProDH activity can be characterized by measuring the rate of absorbance decrease at 600 nm. |
| Synonyms | ProDH |
| Sample Type | Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (580-620 nm, optimum wavelength: 600 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.04 U/L |
| Detection Range | 0.04-1.10 U/L |
| Precision | inter-assay CV: 8.9-10.0% | intra-assay CV: 3.0-3.9% |
| Sample Volume | 40 μL(tissue homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Green leaves tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Garlic tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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