Protein Carbonyl Colorimetric Assay Kit (Tissue and Serum Samples) (E-BC-K117-M)
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For research use only.
| Detection Principle | The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly. |
| Sample Type | Serum,Plasma,Hydrothorax,Cell culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (360-385 nm,optimum wavelength: 370 nm) |
| Research Area | Oxidative Stress , Amino Acids And Proteins |
| Other Reagents Required | Absolute ethanol, Ethyl acetate |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 8.5% | intra-assay CV: 5.2% |
| Sample Volume | 0.2 mL |
| Assay Time | 2 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 8-10 |
| Mouse serum | 8-10 |
| 10% Rat liver tissue homogenate | 2-3 |
| 10% Mouse brain tissue homogenate | 1 |
| Human milk | 1 |
| Human urine | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% fish tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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