Protein Carbonyl Colorimetric Assay Kit (Tissue and serum samples)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K117-S

      Size:
      • 50 Assays
      • 100 Assays
      Qty:
      - +
      Price: $240

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      The reactive oxygen species produced by the aerobic metabolism in the body can cause the oxidation of DNA, lipid and protein. The secondary reaction of the amino acid side chain of the protein with the lipid oxidation product is the main cause of the formation of the carbonyl. Carbonyl is a biological marker of ROS-mediated protein oxidation.

      The key point

      1. When washing the precipitate with anhydrous ethanol-ethyl acetate mixture application solution, the vortex must be voilent the mixing time should not be less than 1 min and the precipitate must be washed to white. If the precipitate still appear yellow, increase the washing times properly of anhydrous ethanol-ethyl acetate mixture application solution to ensure the washing process is sufficient. Otherwise the result will be higher.

      2. The speed of centrifuge should not be reduced, otherwise the result will be higher.

      3.  It is recommended that the round bottom test tube instead of the tip bottom tube should be used to ensure fully washing of the precipitate.

      4.  The protein content of the samples can’t be determined using the coomassie brilliant blue method.

      5. The protein content of the samples should be ranged from 1-10 mg/mL.

      Operation procedures

      Operation table

       

      Sample tube

      Control tube

      Sample (mL)

      0.1

      0.1

      Reagent 3 (mL)

      0.4

       

      Reagent 4 (mL)

       

      0.4

      Mix fully by swirling for 1 min, react with shading light at 37 for 30 min accurately.

      Reagent 5 (mL)

      0.5

      0.5

      Mix fully by swirling for 1 min, centrifuge at 13780 g for 10 min at 4, discard the supernatant and keep the precipitate.

      Anhydrous ethanol-ethyl acetate mixture application solution (mL)

      1.0

      1.0

      Mix fully by swirling for 1 min, centrifuge at 13780 g for 10 min at 4, discard the supernatant and keep the precipitate.

      Anhydrous ethanol-ethyl acetate mixture application solution (mL)

      1.0

      1.0

      Mix fully by swirling for 1 min, centrifuge at 13780 g for 10 min at 4, discard the supernatant and keep the precipitate.

      Anhydrous ethanol-ethyl acetate mixture application solution (mL)

      1.0

      1.0

      Mix fully by swirling for 1 min, centrifuge at 13780 g for 10 min at 4, discard the supernatant and keep the precipitate.

      Anhydrous ethanol-ethyl acetate mixture application solution (mL)

      1.0

      1.0

      Mix fully by swirling for 1 min, centrifuge at 13780 g for 10 min at 4, discard the supernatant and keep the precipitate.

      Reagent 6 (mL)

      1.25

      1.25

      Mix fully and incubate at 37 water bath for 15 min accurately.

      Mix fully by swirling to dissolve the precipitate fully. Centrifuge at 13780 g for 15 min at 4℃, then take the supernatant. Set to zero with reagent 6 and measure the OD values of each tube at 370 nm with 0.5 cm diameter quartz cuvette. Meanwhile, determine the protein concentration of supernatant (E-BC-K318-M, E-BC-K165-S).

      Performance characteristics

      Technical parameter

      Detection range Average inter-assay CV 8.6%
      Sensitivity Average intra-assay CV 4.5%
      Average recovery rate 101%
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      • Reviews (0)
      • Q&A (1)
      Q

      a********** Submitted [ Mar 02 2019 ]

      Asked: what is the normal rang value ? I got the value 0.353 . is this wrong ? and why ?

      Reply

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