Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K117-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $260
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(370 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.

      Performance characteristics

      Sample type serum,plasma,hydrothorax,cell culture supernatant,tissue
      Sensitivity 0.02 nmol/mgprot
      Detection range 0.02-10 nmol/mgprot
      Precision Average inter-assay CV: 8.6%Average intra-assay CV: 4.5%
      Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
      Other reagents required Absolute ethanol, Ethyl acetate
      Storage 2-8℃
      Valid period 6 months

      Citations

      1. Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain
        IF: 4.527
        Journal: Ecotoxicology and Environmental Safety(2019)
        DOI: 10.1016/j.ecoenv.2019.109407
        Products: E-BC-K117-S E-BC-K025-S E-BC-K030-M E-BC-K031-S E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
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      Q

      a********** Submitted [ Mar 02 2019 ]

      Asked: what is the normal rang value ? I got the value 0.353 . is this wrong ? and why ?

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