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Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)

  • Cat.No.:E-BC-K117-S

  • Detection instrument: Spectrophotometer(370 nm)

To Purchase E-BC-K117-S

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Detection principle

The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.

Performance characteristics

Sample type Serum,plasma,hydrothorax,cell culture supernatant,tissue
Sensitivity 0.02 nmol/mgprot
Detection range 0.02-10 nmol/mgprot
Detection method Colorimetric method
Assay type Quantitative
Assay time 240 min
Precision Average inter-assay CV: 8.600%Average intra-assay CV: 4.500%
Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Other reagents required Absolute ethanol, Ethyl acetate
Storage 2-8℃
Valid period 12 months

Dilution of sample


It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment. The sample can be detected by this kit when the protein content of samples is ranged from 1-10 mg/mL.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

8-10

Mouse serum

8-10

10% Rat liver tissue homogenization

2-3

10% Mouse heart tissue homogenization

1

10% Mouse brain tissue homogenization

1

Note: The diluent is double distilled water or reagent 1.


Citations

  1. Redox Biology (2021) IF: 11.799
    Lipid peroxidation as a hallmark of severity in COVID-19 patients

    DOI: 10.1016/j.redox.2021.102181

    PMID: 34768063

    Sample: plasma
  2. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Dyslipidemia and Inflammation as Hallmarks of Oxidative Stress in COVID-19: A Follow-Up Study

    DOI: 10.3390/ijms232315350

    Sample: plasma
  3. Pharmaceuticals (2021) IF: 5.863
    Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

    DOI: 10.3390/ph15010016

    Sample: serum
  4. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019) IF: 4.527
    Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain enzyme activity and immunoexpression of astrocyte in male rats

    DOI: 10.1016/j.ecoenv.2019.109407

    PMID: 31279280

    Sample: Tissue homogenate
  5. Food Quality and Safety (2022) IF: 3.102
    Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

    DOI: 10.1093/fqsafe/fyac032

    Sample: liver
  6. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    Sample: Tissue homogenate
  7. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603

    Sample: brain
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

M**********dSubmitted [ Aug 18 2022 ]

Asked: Is it possible to measure virus solution?

Reply

A

adminSubmitted [ Aug 18 2022 ]

Answered: 

Q

D********dSubmitted [ Aug 02 2022 ]

Asked: Is the sample of cytoplasm avaliable?

Reply

A

adminSubmitted [ Aug 02 2022 ]

Answered: Unfortunately, the concentration of protein carbonyl in cytoplasm is too low to be measured with this kit.

Q

a********** Submitted [ Mar 02 2019 ]

Asked: what is the normal rang value ? I got the value 0.353 . is this wrong ? and why ?

Reply

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