Total Carbonyl Colorimetric Assay Kit (E-BC-K171-S)
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For research use only.
| Detection Principle | Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value. |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (370 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.94 μg/mL |
| Detection Range | 0.94-45 μg/mL |
| Precision | inter-assay CV: 8.3% | intra-assay CV: 4.4% |
| Sample Volume | 0.12 mL |
| Assay Time | 50 min |
| Cat.No. | Product Name | Clone No. |
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