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Protein Carbonyl Colorimetric Assay Kit (Tissue and Serum Samples)

Cat:E-BC-K117-M Citations (2)
Manual MSDS

Price: $ 280

Price: $ 0.01

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;hydrothorax;cell culture supernatant;tissue
  • Detection Instrument: Microplate reader(370 nm)
  • Reasearch Areas: Oxidative stress;Amino acids proteins
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Product Details
Detection Principle The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Sample Type serum,plasma,hydrothorax,cell culture supernatant,tissue
Detection Method Colorimetric method
Assay Time 140 min
Precision inter-assay CV: 8.5% | intra-assay CV: 5.2%
Other Reagents Required Absolute ethanol, Ethyl acetate
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 8-10
Mouse serum 8-10
10% Rat liver tissue homogenate 2-3
10% Mouse brain tissue homogenate 1
Human milk 1
Human urine 1
10% Mouse heart tissue homogenate 1
10% fish tissue homogenate 1
The diluent is homogenate medium. For the dilution of other sample types, please do pretest to confirm the dilution factor.