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Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)

  • Cat.No.:E-BC-K117-M

  • Detection instrument: Microplate reader(370 nm)

To Purchase E-BC-K117-M

  • 96T
Price: $280

Detection principle

The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.

Performance characteristics

Sample type serum,plasma,hydrothorax,cell culture supernatant,tissue
Detection method Colorimetric method
Assay type Quantitative
Assay time 140 min
Precision Average inter-assay CV: 8.5%Average intra-assay CV: 5.2%
Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Other reagents required Absolute ethanol, Ethyl acetate
Storage 2-8℃
Valid period 6 months


The level of carbonyl was significantly increased (P<0.05) in DMH treated groups (G3) comparing to control group (G1). Quercetin administration has inhibitory effects on protein carbonyl level in different phase (G4-G6).

S G Darband et al found that quercetin attenuated oxidative DNA damage in rats with DMH induced colon carcinogenesis, changes of markers of protein oxidation can be obtained. Protein carbonyl level of rat colon tissue was determined using protein carbonyl colorimetric assay kit (E-BC-K117-M).

Ralated product

Cat Number Product Name
E-BC-K171-M Total Carbonyl Colorimetric Assay Kit


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  • Q&A

D********dSubmitted [ Aug 05 2022 ]

Asked: I found that there are E-BC-K117-M and E-BC-K117-S for protein carbonyl, what\'s the relationship or difference between them?



adminSubmitted [ Aug 05 2022 ]

Answered: These two kits are mainly the difference between the detection instruments. One is the detection of the microplate reader, and the other is the detection of the spectrophotometer.You can check our manuals.

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