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Proteinase K Reagent

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      Catalog number:E-IR-R109

      Size:
      • 1mL
      • 5mL
      • 10mL
      Qty:
      - +
      Price: $20

      Application: IF,FCM

      Storage: Store at -20°C. Valid for 12 months.

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

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      Introduction

      Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.

      Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity.

      The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0.

      The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55 °C.


      Components

      Cat.

      Products

      1 mL

      5 mL

      10 mL

      Storage

      E-IR-R109

      Proteinase K(10 mg/mL)

      1 mL

      5 mL

      10 mL

      -20°C

      Manual

       One Copy



      Specification parameters

      Source

      Yeast

      Appearance

      Clear liquid

      MW.

      29 kDa

      CAS NO.

      39450-01-6

      Purity

      95% by SDS-PAGE

      E.C

      3.4.21.64

      Activity

      30U/mg

      DNase

      Free

      Buffer

      PBS with 10mM Tris-HClpH 7.5,5mM CaCl 2 and 50% glycerin

      RNase

      Free




      Storage

      Store at -20°C. Valid for 12 months.

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