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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Human CD22 Antibody[HIB22]

Purified Anti-Human CD22 Antibody[HIB22]

Uniprot : P20273

  • Cat.No.:E-AB-F1321A

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1321A

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  • 25μg
  • 100μg
Price: $43
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Antigen Details

Alternate Names B-cell receptor CD22,Cd22,B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14,CD22,Lyb-8, Siglec2
Swissprot P20273
Gene ID 933
References
1. Clark E. 1993. J. Immunol. 150:4715.
2. Shan D, et al. 1995. J. Immunol. 154:4466.

Product Details

Form Liquid
Clone No. HIB22(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793A]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Unconjugated

How to select the appropriate detection channel through the spectrogram?

Background

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

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