Alternate Names	Interleukin-4,IL-4,B-cell IgG differentiation factor,B-cell growth factor 1,BSF-1,IGG1 induction factor
Swissprot	P05112
References	1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859.

Product Details

Form	Liquid
Clone No.	MP4-25D2(See Other Available Formats)
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	Purified Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823A]
Reactivity	Human
Application	ICFCM,ELISA (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer.
Recommended Use	Each lot of this antibody is quality control tested by ELISA assay. Purified MP4-25D2 has been tested by blocking fluorochrome conjugated MP4-25D2 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 µg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Unconjugated

How to select the appropriate detection channel through the spectrogram?

Background

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Protocols & Troubleshootings

Related Protocols	Staining Intracellular Antigens for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human IL-4 Antibody[MP4-25D2]	50μg, 500μg, 1mg	Check More
FITC Anti-Human IL-4 Antibody[MP4-25D2]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human IL-4 Antibody[MP4-25D2]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human IL-4 Antibody[MP4-25D2]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human IL-4 Antibody[MP4-25D2]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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