Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Mouse CD117/c-Kit Antibody[2B8]
Cat.No.:E-AB-F1092A
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Alternate Names | Mast/stem cell growth factor receptor Kit,Kit,SCFR,Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,CD117 |
Swissprot | P05532 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5. |
Form | Liquid |
Clone No. | 2B8(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843A] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
How to select the appropriate detection channel through the spectrogram?
CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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