Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Mouse H-2 Antibody[M1/42] E-AB-F1216A
Cat.No.:E-AB-F1216A
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Alternate Names | Mouse major histocompatibility complex (MHC) H-2, MHC I |
References |
1. Stallcup KC, et al. 1981. J. Immunol. 127:923.
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Form | Liquid |
Clone No. | M1/42(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833A] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | PBS with 0.05% Proclin300 |
Recommended Use | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 μg per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8℃. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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