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Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Cat.No.:E-BC-K650-M
Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

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96T

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Detection principle

Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms	PDH
Sample type	serum (plasma), urine, animal tissue,cell
Sensitivity	0.28 U /L
Detection range	0.28-76.23 U/L
Assay type	Enzyme Activity
Assay time	15 min
Precision	Average inter-assay CV: 10%Average intra-assay CV: 2%
Other instruments required	Centrifuge, 37℃ incubator
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K652-M	Acetyl-CoA Colorimetric Assay Kit
E-BC-K130-M	Pyruvic Acid Colorimetric Assay Kit

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Collection and treatment of common samples for metabolism assay detection

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