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Rat Aβ1-40(Amyloid Beta 1-40) ELISA Kit

  • Cat.No.:E-EL-R3030

  • Reactivity: Rat

To Purchase E-EL-R3030

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat Aβ1-40 in samples. No significant cross-reactivity or interference between Rat Aβ1-40 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat Aβ1-40 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Aβ1-40. You can calculate the concentration of Rat Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Aβ1-40 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Aβ1-40 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 43.79 115.21 417.92 40.74 106.44 457.42
Standard deviation 2.35 5.66 22.19 2.06 4.96 22.69
CV (%) 5.37 4.91 5.31 5.06 4.66 4.96

Recovery

The recovery of Rat Aβ1-40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 100
EDTA plasma (n=8) 97-108 101
Cell culture media (n=8) 94-104 97

Linearity

Samples were spiked with high concentrations of Rat Aβ1-40 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsAβ40,Amyloid Beta 40

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Brain Behavior and Immunity (2015) IF: 5.964
    Chronic Alpha-Linolenic Acid Treatment Alleviates Age-Associated Neuropathology: Roles Of PERK/eIF2αSignaling Pathway.

    DOI: 10.1016/j.bbi.2015.09.012

    PMID: 26399745

    Sample: Tissue homogenate
  2. Frontiers In Aging Neuroscience (2017) IF: 4.504
    Sex Differences In the Cognitive And Hippocampal Effects Of Streptozotocin In An Animal Model Of Sporadic AD.

    DOI: 10.3389/fnagi.2017.00347

    PMID: 29163130

    Sample: Tissue homogenate
  3. International Journal of Neuropsychopharmacology IF: 3.98
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    Sample: Serum, Tissue homogenate
  4. Journal of Alzheimers Disease (2014) IF: 3.731
    Region-Specific Expression Of Tau, Amyloid-Β Protein Precursor, And Synaptic Proteins At Physiological Condition Or Under Endoplasmic Reticulum Stress In Rats.

    DOI: 10.3233/JAD-140207

    PMID: 24787918

    Sample: Tissue homogenate
  5. Biomedicine & Pharmacotherapy (2017) IF: 2.759
    Thymoquinone-rich Fraction Nanoemulsion (TQRFNE) Decreases Aβ40 And Aβ42 Levels By Modulating APP Processing, Up-regulating IDE and LRP1, And Down-regulating BACE1 And RAGE In Response To High Fat/Cholesterol Diet-induced Rats.

    DOI: 10.1016/j.biopha.2017.08.074

    PMID: 28892789

    Sample: Tissue homogenate
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