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Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit

  • Cat.No.:E-EL-R1430

  • Reactivity: Rat

To Purchase E-EL-R1430

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Ang-Ⅱ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Ang-Ⅱ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.80 133.70 360.10 46.10 133.40 382.00
Standard deviation 3.00 7.60 13.00 3.20 7.10 15.70
CV (%) 6.41 5.68 3.61 6.94 5.32 4.11

Recovery

The recovery of Ang-Ⅱ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma(n=8) 90-104 96
Cell culture media(n=8) 84-96 91

Linearity

Samples were spiked with high concentrations of Rat Ang-Ⅱ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsAngiotensin-2
Research AreaCardiovascular

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. IF: 5.099
    COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism.

    DOI: 10.1016/j.envpol.2018.05.034

    PMID: 29793105

    Sample: Tissue homogenate
  2. Frontiers in Pharmacology (2020) IF: 4.225
    Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

    DOI: 10.3389/fphar.2020.00094

    PMID: 32153406

    Sample: Serum
  3. Toxicology and applied pharmacology (2019) IF: 3.616
    Metformin attenuates cardiovascular and renal injury in uninephrectomized rats on DOCA-salt: Involvement of AMPK and miR-As in cardioprotection

    DOI: 10.1016/j.taap.2018.10.004

    PMID: 30290168

    Sample: Plasma
  4. Molecules (2017) IF: 3.098
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  5. Journal of Translational Medicine (2016) IF: 3.786
    Modulation Of The Sympathetic Nervous System By Renal Denervation Prevents Reduction Of Aortic Distensibility In Atherosclerosis Prone ApoE-Deficient Rats.

    DOI: 10.1186/s12967-016-0914-9

    PMID: 27277003

    Sample: Tissue homogenate
  6. Experimental and Clinical Endocrinology & Diabetes (2019) IF: 2.058
    Hyperuricemia Inhibition Protects SD Rats Against Fructose-Induced Obesity Hypertension Via Modulation of Inflammation and Renin-Angiotensin System in Adipose Tissue

    DOI: 10.1055/a-1023-6710

    PMID: 31683330

    Sample: Serum
  7. Journal of Pharmacy and Pharmacology (2019) IF: 2.571
    The effect of eplerenone on the renin-angiotensin-aldosterone system of rats with thyroid dysfunction

    DOI: 10.1111/jphp.13168

    PMID: 31579950

    Sample: Serum
  8. BMC complementary and alternative medicine (2019) IF: 2.109
    Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats

    DOI: 10.1186/s12906-019-2463-2

    PMID: 30819180

    Sample: Serum
  9. Environmental Toxicology & Pharmacology (2018) IF: 2.331
    The probable roles of valsartan in alleviating chronic obstructive pulmonary disease following co-exposure to cold stress and fine particulate matter.

    DOI: 10.1016/j.etap.2018.05.002

    PMID: 29775776

    Sample: Tissue homogenate
  10. PloS one (2017) IF: 2.806
    Dysregulation Of MicroRNAs And Renin-Angiotensin System In High Salt Diet-Induced Cardiac Dysfunction In Uninephrectomized Rats.

    DOI: 10.1371/journal.pone.0180490

    PMID: 28727756

    Sample: Plasma
  11. Molecules (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  12. European Journal of Pharmacology (2016) IF: 2.896
    The Protective Role Of Neuregulin-1: A Potential Therapy For Sepsis-Induced Cardiomyopathy.

    DOI: 10.1016/j.ejphar.2016.06.042

    PMID: 27346832

    Sample: Serum
  13. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system

    DOI: 10.3892/etm.2020.8759

    PMID: 32742325

    Sample: Cell lysate
  14. Biological and Pharmaceutical Bulletin (2018) IF: 1.54
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    Sample: Plasma
  15. Clinical and Experimental Hypertension (2019) IF: 1.367
    Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats

    DOI: 10.1080/10641963.2018.1506467

    PMID: 30648895

    Sample: Serum
  16. Experimental and therapeutic medicine (2018) IF: 1.41
    Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin-angiotensin system activity

    DOI: 10.3892/etm.2018.6734

    PMID: 30402152

    Sample: Plasma,Tissue homogenate
  17. Journal of Complementary and Integrative Medicine (2020)
    Senecio serratuloides extract prevents the development of hypertension, oxidative stress and dyslipidemia in nitric oxide-deficient rats

    DOI: 10.1515/jcim-2018-0073

    PMID: 32045353

    Sample: Serum
  18. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology (2020)
    Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome

    DOI: 10.4196/kjpp.2020.24.4.319

    PMID: 32587126

    Sample: Serum
  19. IOP Conference Series: Materials Science and Engineering. IOP Publishing (2018)
    Research on the evaluation indexes of animal model of liver-fire hypertension syndrome

    DOI: 10.1088/1757-899X/397/1/012132

    PMID: 99999999

    Sample: Serum
  20. DEStech Transactions on Biology and Health (2018)
    Experimental Study on the Effect of Shugan Jianghuo Granule on Related Indexes of Rats with Liver-fire Hypertension Syndrome

    DOI: 10.12783/dtbh/icmsb2018/25472

    PMID: 99999999

    Sample: Serum
  21. DEStech Transactions on Biology and Health (2018)
    Experimental Study on the Effect of Shugan Jianghuo Granule on Hypertensive Rats with Hyperactivity of Liver Fire

    DOI: 10.12783/dtbh/icmsb2018/25471

    PMID: 99999999

    Sample: Serum
  22. Bangladesh Journal of Pharmacology (2018) IF: 0.814999999
    Mechanism of the diuretic activity of Descurainia sophia seed.

    DOI: 10.3329/bjp.v13i2.35931

    Sample: Urine
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