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Rat cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

  • Cat.No.:E-EL-R0151

  • Reactivity: Rat

To Purchase E-EL-R0151

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Rat cTnT/TNNT2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat cTnT/TNNT2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat cTnT/TNNT2. You can calculate the concentration of Rat cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat cTnT/TNNT2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat cTnT/TNNT2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 53.47 114.59 416.54 57.54 117.04 389.47
Standard deviation 2.78 6.38 22.16 3.02 6.31 17.92
CV (%) 5.20 5.57 5.32 5.25 5.39 4.60


The recovery of Rat cTnT/TNNT2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-97 91
EDTA plasma (n=8) 95-108 100
Cell culture media (n=8) 89-102 95


Samples were spiked with high concentrations of Rat cTnT/TNNT2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCMH2, CMPD2, LVNC6, RCM3, TnTC
Research AreaCancer, Cardiovascular, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


    Hezi inhibits Tiebangchui-induced cardiotoxicity and preserves its anti-rheumatoid arthritis effects by regulating the pharmacokinetics of aconitine and deoxyaconitine

    DOI: 10.1016/j.jep.2022.115915

    Sample: Serum
  2. Computational and Structural Biotechnology Journal (2020) IF: 6.018
    Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

    DOI: 10.1016/j.csbj.2020.11.009

    PMID: 33304464

    Sample: Blood
  3. Chinese Journal of Integrative Medicine (2019) IF: 1.445
    Danshensu Ameliorates Cardiac Ischaemia Reperfusion Injury through Activating Sirt1/FoxO1/Rab7 Signal Pathway

    DOI: 10.1007/s11655-019-3165-9

    Sample: Heart
  4. Molecular Medicine Reports (2015) IF: 1.554
    Candesartan ameliorates acute myocardial infarction in rats through inducible nitric oxide synthase, nuclear factor‑κB, monocyte chemoattractant protein‑1, activator protein‑1 and restoration of heat shock protein 72

    DOI: 10.3892/mmr.2015.4432

    Sample: Serum
  5. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022) IF: 2.01
    Carnosic Acid Protects Against Myocardial Infarction by Controlling Oxidative Stress and Inflammation in Rats

    DOI: 10.1007/s43450-021-00216-8

    Sample: Serum
  6. BMC Cardiovascular Disorders (2022) IF: 2.174
    Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

    DOI: 10.1186/s12872-022-02758-8

    PMID: 35918636

    Sample: Heart Tissue
    Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats

    DOI: 10.1080/15376516.2018.1542474

    Sample: Heart Tissue
  8. BMC Cardiovascular Disorders (2022) IF: 2.298
    H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure

    DOI: 10.1186/s12872-022-02621-w

    PMID: 35439934

    Sample: Serum
    Impact of prolonged nicotine administration on myocardial function and susceptibility to ischaemia‐reperfusion injury in rats

    DOI: 10.1111/bcpt.13500

    Sample: Plasma
    The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy

    DOI: 10.1016/j.ejphar.2016.06.042

    Sample: Serum
  11. Cardiovascular Toxicology (2018) IF: 2.989
    Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction

    DOI: 10.1007/s12012-018-9478-7

    Sample: Plasma
  12. NUTRITION RESEARCH (2022) IF: 3.315
    Inhibition of fat accumulation, lipid dysmetabolism, cardiac inflammation, and improved NO signalling mediate the protective effects of lycopene against cardio-metabolic disorder in obese female rats

    DOI: 10.1016/j.nutres.2022.05.009

    Sample: Serum
  13. Frontiers in Pharmacology (2019) IF: 3.845
    Angiotensin II Type I Receptor Antagonism Attenuates Nicotine-Induced Cardiac Remodeling, Dysfunction, and Aggravation of Myocardial Ischemia-Reperfusion Injury in Rats

    DOI: 10.3389/fphar.2019.01493

    Sample: Heart
  14. Scientific Reports (2021) IF: 4.38
    Targeting mitochondrial reactive oxygen species-mediated oxidative stress attenuates nicotine-induced cardiac remodeling and dysfunction

    DOI: 10.1038/s41598-021-93234-4

    PMID: 34226619

    Sample: Heart
  15. MOLECULES (2021) IF: 4.412
    Comparative Protective Effect of Nigella sativa Oil and Vitis vinifera Seed Oil in an Experimental Model of Isoproterenol-Induced Acute Myocardial Ischemia in Rats

    DOI: 10.3390/molecules26113221

    PMID: 34072098

    Sample: Serum
  16. FASEB JOURNAL (2019) IF: 5.391
    Physiological levels of chromogranin A prevent doxorubicin-induced cardiotoxicity without impairing its anticancer activity

    DOI: 10.1096/fj.201802707R

    Sample: Plasma
  17. Frontiers in Oncology (2022) IF: 5.738
    Molecular Changes In Cardiac Tissue As A New Marker To Predict Cardiac Dysfunction Induced By Radiotherapy

    DOI: 10.3389/fonc.2022.945521

    Sample: Plasma
  18. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats

    DOI: 10.1155/2022/1090893

    Sample: Serum
  19. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    An Energy Metabolism Study on the Efficacy of Naoxintong Capsules against Myocardial Infarction in a Rat Model

    DOI: 10.1155/2022/3712500

    PMID: 35915610

    Sample: Serum
    Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis

    DOI: 10.1016/j.biopha.2022.113437

    PMID: 36076489

    Sample: Serum
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