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Rat Cys-C(Cystatin C) ELISA Kit

  • Cat.No.:E-EL-R0304

  • Reactivity: Rat

To Purchase E-EL-R0304

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat Cys-C in samples. No significant cross-reactivity or interference between Rat Cys-C and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat Cys-C concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Cys-C. You can calculate the concentration of Rat Cys-C in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Cys-C were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Cys-C were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.10 4.00 0.50 1.10 4.20
Standard deviation 0.03 0.05 0.20 0.03 0.06 0.15
CV (%) 6.00 4.55 5.00 6.00 5.45 3.57


The recovery of Rat Cys-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 96
EDTA plasma (n=8) 86-99 91
Cell culture media (n=8) 93-107 101


Samples were spiked with high concentrations of Rat Cys-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCST3, Cystatin 3, ARMD11, Post-Gamma-Globulin
Research AreaCancer, Cell Biology, Cardiovascular, Stem cells, Tags and Cell Markers

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Computational and Structural Biotechnology Journal (2020) IF: 6.018
    Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

    DOI: 10.1016/j.csbj.2020.11.009

    PMID: 33304464

    Sample: blood
    Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms

    DOI: 10.1016/j.ejmech.2021.113388

    PMID: 33784603

    Sample: serum
  3. LIFE SCIENCES (2022) IF: 5.037
    Angiotensin 1–7 mitigates rhabdomyolysis induced renal injury in rats via modulation of TLR-4/NF-kB/iNOS and Nrf-2/heme‑oxygenase-1 signaling pathways

    DOI: 10.1016/j.lfs.2022.120678

    Sample: serum
  4. Cell Death Discovery (2022) IF: 5.241
    Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats

    DOI: 10.1038/s41420-022-00822-y

    PMID: 35022384

    Sample: serum
  5. Drug Design Development and Therapy (2022) IF: 4.319
    Alpha-Chymotrypsin Protects Against Acute Lung, Kidney, and Liver Injuries and Increases Survival in CLP-Induced Sepsis in Rats Through Inhibition of TLR4/NF-κB Pathway

    DOI: 10.2147/DDDT.S370460

    Sample: serum
    Nephroprotective mechanisms of Ambrette (Abelmoschus moschatus Medik.) leaf extracts in adriamycin mediated acute kidney injury model of Wistar rats

    DOI: 10.1016/j.jep.2022.115221

    PMID: 35339624

    Sample: Kidney
    Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy

    DOI: 10.1159/000452561

    Sample: Plasma,Tissue homogenate
  8. Biomedicines (2022) IF: 4.757
    Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study

    DOI: 10.3390/biomedicines10071613

    PMID: 35884918

    Sample: Serum
    Protective effect of empagliflozin on gentamicin-induced acute renal injury via regulation of SIRT1/NF-κB signaling pathway

    DOI: 10.1016/j.etap.2022.103907

    PMID: 35697188

    Sample: serum
  10. Toxicology Research (2022) IF: 3.524
    Cerium oxide nanoparticles attenuate the renal injury induced by cadmium chloride via improvement of the NBN and Nrf2 gene expressions in rats

    DOI: 10.1093/toxres/tfac009

    PMID: 35510236

    Sample: kidney
    Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress

    DOI: 10.1093/jpp/rgab091

    Sample: serum
    Angiotensin aldosterone inhibitors improve survival and ameliorate kidney injury induced by sepsis through suppression of inflammation and apoptosis

    DOI: 10.1111/fcp.12718

    PMID: 34309069

    Sample: serum
  13. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Mechanism of myocardial ischemia/reperfusion‑induced acute kidney injury through DJ‑1/Nrf2 pathway in diabetic rats

    DOI: 10.3892/etm.2017.5095

    Sample: Serum
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    Sample: Plasma
  15. RENAL FAILURE (2015) IF: 0.944
    The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats

    DOI: 10.3109/0886022X.2015.1120119

    Sample: Serum
  16. Acta Cirurgica Brasileira (2020) IF: 0.974
    Effects of ATRA on diabetic rats with renal ischemia-reperfusion injury

    DOI: 10.1590/s0102-865020200010000006

    PMID: 32236320

    Sample: Serum
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