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Rat GC(Glucagon) ELISA Kit

  • Cat.No.:E-EL-R0425

  • Reactivity: Rat

To Purchase E-EL-R0425

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GC in samples. No significant cross-reactivity or interference between GC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GC concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GC. During the reaction, GC in the sample or standard competes with a fixed amount of GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GC were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GC were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 205.30 401.50 1351.70 185.10 391.80 1483.10
Standard deviation 11.70 17.30 58.10 9.80 18.00 60.80
CV (%) 5.70 4.31 4.30 5.29 4.59 4.10


The recovery of GC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 98
EDTA plasma(n=8) 92-104 98
Cell culture media(n=8) 95-106 101


Samples were spiked with high concentrations of Rat GC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Computational and Structural Biotechnology Journal (2020) IF: 6.018
    Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

    DOI: 10.1016/j.csbj.2020.11.009

    PMID: 33304464

    Sample: plasma
    Metabolic and Molecular Response to High-Fat Diet Differs between Rats with Constitutionally High and Low Serotonin Tone

    DOI: 10.3390/ijms24032169

    Sample: plasma
  3. Antioxidants (2022) IF: 6.313
    β-Sitosterol Glucoside-Loaded Nanosystem Ameliorates Insulin Resistance and Oxidative Stress in Streptozotocin-Induced Diabetic Rats

    DOI: 10.3390/antiox11051023

    Sample: plasma
    Kynurenine-3-monooxygenase expression is activated in the pancreatic endocrine cells by diabetes and its blockade improves glucose-stimulated insulin secretion

    DOI: 10.1016/j.bbadis.2022.166509

    PMID: 35914653

    Sample: islet
    Pancreastatin inhibitor PSTi8 ameliorates streptozotocin-induced diabetes by suppressing hepatic glucose production

    DOI: 10.1016/j.ejphar.2023.175559

    Sample: plasma
    Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis

    DOI: 10.3390/ijms22105400

    PMID: 34065591

    Sample: Plasma
  7. Frontiers in Neural Circuits (2021) IF: 3.492
    Excitatory Impact of Dental Occlusion on Dorsal Motor Nucleus of Vagus

    DOI: 10.3389/fncir.2021.638000

    PMID: 33776655

    Sample: Serum
  8. African Journal of Traditional Complementary and Alternative Medicines (2016) IF: 0.553
    Asiatic acid influences glucose homeostasis in P. berghei murine malaria infected sprague-dawley rats

    DOI: 10.4314/ajtcam.v13i5

    Sample: plasma
  9. Ulusal Travma ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery (2022) IF: 0.894
    Intraductal botulinum toxin injection suppressed the inflammation in experimental acute pancreatitis.

    DOI: 10.14744/tjtes.2021.90140

    Sample: blood
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