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Rat GHRL(Ghrelin) ELISA Kit

  • Cat.No.:E-EL-R0842

  • Reactivity: Rat

To Purchase E-EL-R0842

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GHRL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GHRL. During the reaction, GHRL in the sample or standard competes with a fixed amount of GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GHRL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GHRL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 0.90 3.80 0.50 0.90 4.00
Standard deviation 0.03 0.05 0.13 0.03 0.04 0.20
CV (%) 6.00 5.56 3.42 6.00 4.44 5.00


The recovery of GHRL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma(n=8) 85-101 92
Cell culture media(n=8) 93-106 98


Samples were spiked with high concentrations of Rat GHRL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsObestatin Preprohormone, MTLRP, Ghrelin
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


  1. Vascular Health and Risk Management (2023)
    The Effectiveness of Nigella sativa and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats

    DOI: 10.2147/VHRM.S396295

    Sample: Serum
  2. Scientific African (2021)
    Effect of Allium sativum extract in combination -with orlistat on insulin resistance and disrupted metabolic hormones in high fat diet induced obese rats

    DOI: 10.1016/j.sciaf.2021.e00994

    Sample: Serum
  3. Open Life Sciences (2022) IF: 1.311
    Physical activity enhances fecal lactobacilli in rats chronically drinking sweetened cola beverage

    DOI: 10.1515/biol-2022-0070

    Sample: Plasma
  4. BIOLOGIA (2022) IF: 1.653
    The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model

    DOI: 10.1007/s11756-022-01266-6

    Sample: Serum
    Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones

    DOI: 10.1080/10520295.2021.1896780

    PMID: 33722110

    Sample: Serum
    Evaluation of the effects of bredemolic acid on selected markers of glucose homeostasis in diet-induced prediabetic rats

    DOI: 10.1080/13813455.2019.1680697

    Sample: Plasma
    The effects of intracerebroventricular infusion of irisin on feeding behaviour in rats

    DOI: 10.1016/j.neulet.2017.02.066

    Sample: Serum
  8. Integrative Medicine Research (2019) IF: 2.379
    Orthosiphon stamineus as a potential antidiabetic drug in maternal hyperglycemia in streptozotocin-induced diabetic rats

    DOI: 10.1016/j.imr.2019.05.006

    Sample: Plasm
  9. MOLECULES (2018) IF: 3.098
    The Effects of Plant-Derived Oleanolic Acid on Selected Parameters of Glucose Homeostasis in a Diet-Induced Pre-Diabetic Rat Model

    DOI: 10.3390/molecules23040794

    Sample: Plasma
  10. Scientific Reports (2020) IF: 3.998
    Effect of different levels of hydrolysable tannin intake on the reproductive hormones and serum biochemical indices in healthy female rats

    DOI: 10.1038/s41598-020-77672-0

    PMID: 33244120

    Sample: Serum
  11. Scientific Reports (2021) IF: 4.38
    Acetate rescues defective brain-adipose metabolic network in obese Wistar rats by modulation of peroxisome proliferator-activated receptor-γ

    DOI: 10.1038/s41598-021-98605-5

    PMID: 34556775

    Sample: Plasma
    Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

    DOI: 10.1016/j.psyneuen.2022.105862

    PMID: 35835020

    Sample: Serum
  13. Food & Function (2022) IF: 5.396
    Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

    DOI: 10.1039/D1FO01744F

    PMID: 35044398

    Sample: Serum
  14. CARBOHYDRATE POLYMERS (2022) IF: 9.381
    Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota

    DOI: 10.1016/j.carbpol.2022.119304

    PMID: 35422283

    Sample: Serum
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