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Rat GPX1(Glutathione Peroxidase 1) ELISA Kit

Citations(30)Uniprot : P04041

  • Cat.No.:E-EL-R2491

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat GPX1 in samples. No significant cross-reactivity or interference between Rat GPX1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat GPX1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPX1. You can calculate the concentration of Rat GPX1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GPX1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GPX1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 96.24 211.75 917.74 87.24 225.09 838.83
Standard deviation 6.06 11.96 27.81 4.72 11.77 29.95
CV (%) 6.30 5.65 3.03 5.41 5.23 3.57

Recovery

The recovery of Rat GPX1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 99
EDTA plasma (n=8) 91-105 97
Cell culture media (n=8) 86-100 92

Linearity

Samples were spiked with high concentrations of Rat GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P04041
Research AreaCancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.3389/fmolb.2022.1029729

    PMID: 36330218

    		 Sample: liver
  2. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400
    Sample: kidney
  3. Nutrition & Metabolism (2021) IF: 4.169
    Diet-induced prediabetes: effects of exercise treatment on risk factors for cardiovascular complications

    DOI: 10.1186/s12986-021-00573-0

    PMID: 33888141

    		 Sample: Tissue homogenate(heart)
  4. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Differential neuroprotective effect of curcuminoid formulations in aluminum chloride–induced Alzheimer’s disease

    DOI: 10.1007/s11356-022-20593-4
    Sample: brain
  5. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690
    Sample: skeletal muscle,heart,pancreas
  6. Journal of Diabetes Research (2019) IF: 3.04
    The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers

    DOI: 10.1155/2019/6753541

    PMID: 31828165

    		 Sample: Plasma,Tissue homogenate
  7. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168
    Heamanetic Effects of a Dioxidovanadium(V) Complex in STZ-Induced Diabetic Male Sprague Dawley Rats

    DOI: 10.2147/DMSO.S214726

    PMID: 34707382

    		 Sample: plasma
  8. TOXICOLOGY (2018) IF: 3.265
    Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings

    DOI: 10.1016/j.tox.2018.05.012
    Sample: Tissue homogenate
  9. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  10. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2019) IF: 3.319
    Plant-derived oleanolic acid ameliorates markers associated with non-alcoholic fatty liver disease in a diet-induced pre-diabetes rat model

    DOI: 10.2147/DMSO.S218626
    Sample: plasma
  11. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2019) IF: 3.319
    Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats

    DOI: 10.2147/DMSO.S183811
    Sample: Tissue homogenate,Plasma
  12. DISEASE MARKERS (2021) IF: 3.434
    Protective Effect of Fasudil on Hydrogen Peroxide-Induced Oxidative Stress Injury of H9C2 Cardiomyocytes

    DOI: 10.1155/2021/8177705

    PMID: 34900030

    		 Sample: H9C2 cell
  13. SAUDI PHARMACEUTICAL JOURNAL (2019) IF: 3.643
    Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1

    DOI: 10.1016/j.jsps.2019.11.013

    PMID: 31920438

    		 Sample: Tissue homogenate
  14. LIFE SCIENCES (2020) IF: 3.647
    l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture

    DOI: 10.1016/j.lfs.2020.117502

    PMID: 32142764

    		 Sample: Cell culture supernatant,Tissue homogenate
  15. LIFE SCIENCES (2020) IF: 3.647
    Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

    DOI: 10.1016/j.lfs.2020.118876

    PMID: 33310035

    		 Sample: cell
  16. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
    The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers

    DOI: 10.1016/j.biopha.2019.108925
    Sample: plasma,kidney tissues
  17. Scientific Reports (2020) IF: 3.998
    The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals

    DOI: 10.1038/s41598-020-72098-0

    PMID: 32929110

    		 Sample: Tissue homogenate
  18. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019) IF: 2.041
    The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2019-0369

    PMID: 31491333

    		 Sample: Serum,Plasma,Tissue homogenate
  19. Canadian Journal of Gastroenterology and Hepatology (2020) IF: 2.055
    Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2475301

    PMID: 32149046

    		 Sample: Tissue homogenate
  20. BMC Cardiovascular Disorders (2022) IF: 2.174
    Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

    DOI: 10.1186/s12872-022-02758-8

    PMID: 35918636

    		 Sample: heart tissue
  21. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2021) IF: 2.273
    Anti-hyperglycaemic effects of dioxidovanadium complex cis-[VO2(obz)py] avert kidney dysfunction in streptozotocin-induced diabetic male Sprague–Dawley rats

    DOI: 10.1139/cjpp-2020-0278
    Sample: kidney
  22. Metabolic Syndrome and Related Disorders (2022) IF: 2.363
    Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine

    DOI: 10.1089/met.2022.0028

    PMID: 35796694

    		 Sample: Liver tissue
  23. BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639
    Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods

    DOI: 10.1007/s12011-020-02249-x

    PMID: 32594358

    		 Sample: Tissue homogenate
  24. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    Neuroprotective effects of Hericium erinaceus (Bull.: Fr.) Pers. against high-dose corticosterone-induced oxidative stress in PC-12 cells

    DOI: 10.1186/s12906-020-03132-x

    PMID: 33176761

    		 Sample: Cell culture supernatant
  25. Brazilian Journal of Pharmaceutical Sciences (2022) IF: 1.214
    Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy

    DOI: 10.1590/s2175-97902022e19652
    Sample: Heart tissue
  26. Molecular Medicine Reports (2015) IF: 1.554
    Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti‑inflammatory effects, and inhibition of the p38‑MAPK mechanism

    DOI: 10.3892/mmr.2015.4425
    Sample: Serum
  27. CURRENT EYE RESEARCH (2020) IF: 1.754
    Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

    DOI: 10.1080/02713683.2020.1805768

    PMID: 32744464

    		 Sample: Tissue homogenate
  28. Brazilian Journal of Otorhinolaryngology (2022) IF: 1.811
    Astaxanthin protects against hearing impairment in diabetic rats

    DOI: 10.1016/j.bjorl.2022.02.004

    PMID: 35331657

    		 Sample: serum,cochlea
  29. MEDICAL SCIENCE MONITOR (2020) IF: 1.918
    Pyrroloquinoline Quinone Inhibits Oxidative Stress in Rats with Diabetic Nephropathy

    DOI: 10.12659/MSM.924372
    Sample: kidney tissue,NRK-52E cell
  30. Advances in Traditional Medicine (2020)
    Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus

    DOI: 10.1007/s13596-020-00449-3
    Sample: Tissue homogenate
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