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Rat GPX1(Glutathione Peroxidase 1) ELISA Kit

  • Cat.No.:E-EL-R2491

  • Reactivity: Rat

To Purchase E-EL-R2491

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat GPX1 in samples. No significant cross-reactivity or interference between Rat GPX1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat GPX1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPX1. You can calculate the concentration of Rat GPX1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GPX1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GPX1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 96.24 211.75 917.74 87.24 225.09 838.83
Standard deviation 6.06 11.96 27.81 4.72 11.77 29.95
CV (%) 6.30 5.65 3.03 5.41 5.23 3.57

Recovery

The recovery of Rat GPX1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 99
EDTA plasma (n=8) 91-105 97
Cell culture media (n=8) 86-100 92

Linearity

Samples were spiked with high concentrations of Rat GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P04041
SynonymsGPX1, GPXD, GSHPX1, glutathione peroxidase 1

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Scientific Reports (2020) IF: 3.998
    The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals

    DOI: 10.1038/s41598-020-72098-0

    PMID: 32929110

    Sample: Tissue homogenate
  2. Life Sciences (2019) IF: 3.647
    l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture

    DOI: 10.1016/j.lfs.2020.117502

    PMID: 32142764

    Sample: Cell culture supernatant,Tissue homogenate
  3. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    Sample: Serum
  4. Toxicology (2018) IF: 3.265
    Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings.

    DOI: 10.1016/j.tox.2018.05.012

    PMID: 29800585

    Sample: Tissue homogenate
  5. Biological Trace Element Research (2020) IF: 2.639
    Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods

    DOI: 10.1007/s12011-020-02249-x

    PMID: 32594358

    Sample: Tissue homogenate
  6. Journal of Diabetes Research (2019) IF: 2.965
    The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers

    DOI: 10.1155/2019/6753541

    PMID: 31828165

    Sample: Plasma,Tissue homogenate
  7. Molecular vision (2019) IF: 2.219
    Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

    DOI: 99999999

    PMID: 30820147

    Sample: Tissue homogenate
  8. Canadian Journal of Gastroenterology and Hepatology (2020) IF: 2.055
    Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2475301

    PMID: 32149046

    Sample: Tissue homogenate
  9. Saudi Pharmaceutical Journal (2020) IF: 2.879
    Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1

    DOI: 10.1016/j.jsps.2019.11.013

    PMID: 31920438

    Sample: Tissue homogenate
  10. Canadian journal of physiology and pharmacology (2019) IF: 1.946
    The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2019-0369

    PMID: 31491333

    Sample: Serum,Plasma,Tissue homogenate
  11. Current Eye Research (2020) IF: 1.754
    Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

    DOI: 10.1080/02713683.2020.1805768

    PMID: 32744464

    Sample: Tissue homogenate
  12. Molecular Medicine Reports (2015) IF: 1.692
    Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism.

    DOI: 10.3892/mmr.2015.4425

    PMID: 26458544

    Sample: Serum
  13. Oriental Pharmacy and Experimental Medicine (2020)
    Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus

    DOI: 10.1007/s13596-020-00449-3

    PMID: 99999999

    Sample: Tissue homogenate
  14. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2019)
    Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats.

    DOI: 10.2147/DMSO.S183811

    PMID: 30858714

    Sample: Tissue homogenate,Plasma
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