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Rat GPX1(Glutathione Peroxidase 1) ELISA Kit

Citations(13)Uniprot : P04041

Cat.No.:E-EL-R2491
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat GPX1 in samples. No significant cross-reactivity or interference between Rat GPX1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat GPX1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPX1. You can calculate the concentration of Rat GPX1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GPX1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GPX1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	96.24	211.75	917.74	87.24	225.09	838.83
Standard deviation	6.06	11.96	27.81	4.72	11.77	29.95
CV (%)	6.30	5.65	3.03	5.41	5.23	3.57

Recovery

The recovery of Rat GPX1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-107	99
EDTA plasma (n=8)	91-105	97
Cell culture media (n=8)	86-100	92

Linearity

Samples were spiked with high concentrations of Rat GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P04041
Research Area	Cancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Scientific Reports (2020) IF: 3.998

The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals

DOI: 10.1038/s41598-020-72098-0
PMID: 32929110	Sample: Tissue homogenate

Life Sciences (2019) IF: 3.647

l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture

DOI: 10.1016/j.lfs.2020.117502
PMID: 32142764	Sample: Cell culture supernatant,Tissue homogenate

Environmental Toxicology and Pharmacology (2020) IF: 3.292

Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

DOI: 10.1016/j.etap.2020.103387
PMID: 32339907	Sample: Serum

Biological Trace Element Research (2020) IF: 2.639

Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods

DOI: 10.1007/s12011-020-02249-x
PMID: 32594358	Sample: Tissue homogenate

Journal of Diabetes Research (2019) IF: 2.965

The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers

DOI: 10.1155/2019/6753541
PMID: 31828165	Sample: Plasma,Tissue homogenate

Molecular vision (2019) IF: 2.219

Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups


PMID: 30820147	Sample: Tissue homogenate

Canadian Journal of Gastroenterology and Hepatology (2020) IF: 2.055

Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model

DOI: 10.1155/2020/2475301
PMID: 32149046	Sample: Tissue homogenate

Saudi Pharmaceutical Journal (2020) IF: 2.879

Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1

DOI: 10.1016/j.jsps.2019.11.013
PMID: 31920438	Sample: Tissue homogenate

Canadian journal of physiology and pharmacology (2019) IF: 1.946

The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

DOI: 10.1139/cjpp-2019-0369
PMID: 31491333	Sample: Serum,Plasma,Tissue homogenate

Current Eye Research (2020) IF: 1.754

Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

DOI: 10.1080/02713683.2020.1805768
PMID: 32744464	Sample: Tissue homogenate

Molecular Medicine Reports (2015) IF: 1.692

Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism.

DOI: 10.3892/mmr.2015.4425
PMID: 26458544	Sample: Serum

Oriental Pharmacy and Experimental Medicine (2020)

Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus

DOI: 10.1007/s13596-020-00449-3
	Sample: Tissue homogenate

Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2019)

Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats.

DOI: 10.2147/DMSO.S183811
PMID: 30858714	Sample: Tissue homogenate,Plasma

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