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Rat IL-10(Interleukin 10) ELISA Kit

  • Cat.No.:E-EL-R0016

  • Reactivity: Rat

To Purchase E-EL-R0016

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-10 in samples. No significant cross-reactivity or interference between Rat IL-10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-10 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-10. You can calculate the concentration of Rat IL-10 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-10 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 94.40 238.30 785.60 92.10 254.40 747.70
Standard deviation 5.90 13.60 23.60 5.20 14.50 32.90
CV (%) 6.25 5.71 3.00 5.65 5.70 4.40

Recovery

The recovery of Rat IL-10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 92
EDTA plasma (n=8) 93-104 98
Cell culture media (n=8) 92-105 98

Linearity

Samples were spiked with high concentrations of Rat IL-10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P29456
SynonymsIL10, IL10A, CSIF, TGIF
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Pineal Research (2014) IF: 10.391
    Melatonin Normalizes Clinical And Biochemical Parameters Of Mild Inflammation In Diet-Induced Metabolic Syndrome In Rats.

    DOI: 10.1111/jpi.12168

    PMID: 25113124

    Sample: Plasma
  2. International journal of biological macromolecules (2020) IF: 5.162
    Anti-inflammatory effect of epidermal growth factor conjugated silk fibroin immobilized polyurethane ameliorates diabetic burn wound healing

    DOI: 10.1016/j.ijbiomac.2019.09.219

    PMID: 31647938

    Sample: Serum,Tissue homogenate
  3. development (2019) IF: 5.413
    Effects of Tarantula cubensis alcoholic extract and Nerium oleander distillate on experimentally induced colon cancer

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  4. Molecular Pharmaceutics (2020) IF: 4.32
    Accelerated Blood Clearance of Nanoemulsions Modified with PEG- Cholesterol and PEG-Phospholipid Derivatives in Rats: The Effect of PEG-Lipid Linkages and PEG Molecular Weights

    DOI: 10.1021/acs.molpharmaceut.9b00770

    PMID: 99999999

    Sample: Plasma,Tissue homogenate
  5. Toxicology (2020) IF: 4.099
    Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats

    DOI: 10.1016/j.tox.2020.152556

    PMID: 32758512

    Sample: Serum,Tissue homogenate
  6. Molecular Pharmaceutics (2019) IF: 4.321
    Accelerated Blood Clearance of Nanoemulsions Modified with PEG-Cholesterol and PEG-Phospholipid Derivatives in Rats: The Effect of PEG-Lipid Linkages and PEG Molecular Weights

    DOI: 10.1021/acs.molpharmaceut.9b00770

    PMID: 31860321

    Sample: Serum
  7. Journal of cellular biochemistry (2019) IF: 4.237
    Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study

    DOI: 10.1002/jcb.28976

    PMID: 31099131

    Sample: Tissue homogenate
  8. Oxidative Medicine and Cellular Longevity (2018) IF: 4.936
    Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways.

    DOI: 10.1155/2018/9647809

    PMID: 29977458

    Sample: Cells
  9. Scientific Reports (2017) IF: 4.259
    Effect Of Niacin On Inflammation And Angiogenesis In A Murine Model Of Ulcerative Colitis.

    DOI: 10.1038/s41598-017-07280-y

    PMID: 28769047

    Sample: Tissue homogenate
  10. Scientific Reports (2017) IF: 4.259
    Hydrogen Sulfide Ameliorates Chronic Renal Failure In Rats By Inhibiting Apoptosis And Inflammation Through ROS/MAPK And NF-κB Signaling Pathways.

    DOI: 10.1038/s41598-017-00557-2

    PMID: 28352125

    Sample: Tissue homogenate
  11. European review for medical and pharmacological sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (miR-2) and miR-101 on sepsis-induced myocardial injury in rats

    DOI: 10.26355/eurrev_202006_21528

    PMID: 99999999

    Sample: Serum
  12. International Immunopharmacology (2020) IF: 3.943
    The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats

    DOI: 10.1016/j.intimp.2020.106615

    PMID: 32447219

    Sample: Plasma
  13. Biology of Reproduction (2019) IF: 3.322
    Changes in alpha-7 nicotinic acetylcholine receptor and macrophage polarization state participate in the regulation of cervical remodeling in pregnant rats?

    DOI: 10.1093/biolre/ioz133

    PMID: 31342065

    Sample: Tissue homogenate
  14. Immunology and cell biology (2019) IF: 3.745
    Effects of IL-10- and FasL-overexpressing dendritic cells on liver transplantation tolerance in a heterotopic liver transplantation rat model

    DOI: 10.1111/imcb.12252

    PMID: 30977930

    Sample: Cell culture supernatant
  15. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Trichosanthes kirilowii lectin ameliorates streptozocin-induced kidney injury via modulation of the balance between M1/M2 phenotype macrophage

    DOI: 10.1016/j.biopha.2018.10.060

    PMID: 30396096

    Sample: Serum
  16. Journal of Cellular Physiology (2019) IF: 3.923
    C1q/TNF-related protein-9 promotes macrophage polarization and improves cardiac dysfunction after myocardial infarction

    DOI: 10.1002/jcp.28513

    PMID: 30953351

    Sample: Tissue homogenate
  17. Physiology & Behavior (2020) IF: 2.826
    Inhibition of the fatty acid amide hydrolase changes behaviors and brain catecholamines in a sex-specific manner in rats exposed to chronic unpredictable stress

    DOI: 10.1016/j.physbeh.2020.113174

    PMID: 32966816

    Sample: Tissue homogenate
  18. Future Microbiology (2020) IF: 2.907
    The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

    DOI: 10.2217/fmb-2020-0005

    PMID: 32250184

    Sample: Serum
  19. Cell Stress and Chaperones (2020) IF: 2.892
    Hydrogen sulfide renal protective effects: possible link between hydrogen sulfide and endogenous carbon monoxide in a rat model of renal injury

    DOI: 10.1007/s12192-019-01055-2

    PMID: 32088905

    Sample: Tissue homogenate
  20. Journal of cellular biochemistry (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    Sample: Serum,Bronchoalveolar Lavage Fluid
  21. American Journal of Reproductive Immunology (2019) IF: 2.745
    Nisin Reduces Uterine Inflammation in Rats by Modulating Concentrations of Pro- And Anti-Inflammatory Cytokines

    DOI: 10.1111/aji.13096

    PMID: 30681748

    Sample: Serum
  22. European Journal of Pharmacology (2017) IF: 2.896
    Vinpocetine Mitigates Proteinuria And Podocytes Injury In A Rat Model Of Diabetic Nephropathy.

    DOI: 10.1016/j.ejphar.2017.08.027

    PMID: 28843828

    Sample: Serum
  23. International Journal of Molecular Medicine (2017) IF: 2.341
    Sirt1 Exerts Neuroprotective Effects By Attenuating Cerebral Ischemia/Reperfusion-Induced Injury Via Targeting P53/Microrna-22.

    DOI: 10.3892/ijmm.2016.2806

    PMID: 27878231

    Sample: Tissue homogenate
  24. Plos One (2015) IF: 2.806
    Extracellular Vesicles Derived From Bone Marrow Mesenchymal Stem Cells Protect Against Experimental Colitis Via Attenuating Colon Inflammation, Oxidative Stress And Apoptosis.

    DOI: 10.1371/journal.pone.0140551

    PMID: 26469068

    Sample: Tissue homogenate
  25. Plos One (2015) IF: 2.806
    Transplantation Of Induced Pluripotent Stem Cells Alleviates Cerebral Inflammation And Neural Damage In Hemorrhagic Stroke.

    DOI: 10.1371/journal.pone.0129881

    PMID: 26086994

    Sample: Tissue homogenate
  26. International Journal of Endocrinology (2015) IF: 2.51
    17β Estradiol Modulates Perfusion Pressure And Expression Of 5-LOX And CYP450 4A In The Isolated Kidney Of Metabolic Syndrome Female Rats.

    DOI: 10.1155/2015/149408

    PMID: 26491436

    Sample: Tissue homogenate
  27. Virus Research (2014) IF: 2.628
    Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.

    DOI: 10.1016/j.virusres.2014.10.011

    PMID: 25451068

    Sample: Tissue homogenate
  28. Experimental and Therapeutic Medicine (2018) IF: 1.261
    Gambogic Acid Exerts Cardioprotective Effects In A Rat Model Of Acute Myocardial Infarction Through Inhibition Of Inflammation, iNOS And NFκB/p38 Pathway.

    DOI: 10.3892/etm.2017.5599

    PMID: 29434760

    Sample: Serum
  29. Toxicology Research (2017) IF: 1.969
    Nano NIO Induced Liver Toxicity Via Activating The NF-κB Signaling Pathway In Rats.

    DOI: 10.1039/c6tx00444j

    PMID: 30090495

    Sample: Tissue homogenate
  30. Immunopharmacology & Immunotoxicology (2016) IF: 1.475
    Anti-Oxidant And Anti-Inflammatory Effects Of Apigenin In A Rat Model Of Sepsis: An Immunological, Biochemical, And Histopathological Study.

    DOI: 10.3109/08923973.2016.1173058

    PMID: 27144896

    Sample: Serum
  31. International Journal of Clinical and Experimental Pathology (2020) IF: 0.252
    Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway

    DOI: 99999999

    PMID: 32509067

    Sample: Serum
  32. Iraqi Journal of Pharmaceutical Sciences (2018)
    Effects of Vitamin E and Q10 Supplementation against Doxorubicin-Induced Neurotoxicity in Rats

    DOI: 10.31351/vol27iss2pp24-31

    PMID: 99999999

    Sample: Tissue homogenate
  33. Asian Hematology Research Journal (2018)
    Does Single/Combined Administration of Tramadol/Viagra Have Reversal Effects on Haematological and Anti-Inflammatory Cytokines in Male Albino Rats?

    DOI: 10.9734/AHRJ/2018/44275

    PMID: 99999999

    Sample: Serum
  34. Indonesian Journal of Tropical and Infectious Disease (2019)
    EFFECT OF AFRICAN LEAF (VERNONIA AMYGDALINA) TO IL-6 AND IL-10 LEVEL ON STAPHYLOCOCCUC AUREUS INFECTION

    DOI: 10.20473/ijtid.v7i4.9654

    PMID: 99999999

    Sample: Whole Blood
  35. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.166
    Effects of methylene blue on the nitric oxide-soluble guanylate cyclase-cyclic guanylyl monophosphate pathway and cytokine levels in rats with sepsis

    DOI: 99999999

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  36. Iranian Journal of Immunology (2016) IF: 0.85
    The Immunostimulatory Effect Of Lactic Acid Bacteria In A Rat Model.

    DOI: IJIv13i3A7

    PMID: 27671513

    Sample: Serum
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