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Current Position:Home>>Kits>>ELISA Kits>> Rat IL-6(Interleukin 6) ELISA Kit

Rat IL-6(Interleukin 6) ELISA Kit

Citations(156)Uniprot : P20607

  • Cat.No.:E-EL-R0015

  • Reactivity: Rat
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Manual MSDS

To Purchase E-EL-R0015

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 12.50-800 pg/mL
Sensitivity 7.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-6 in samples. No significant cross-reactivity or interference between Rat IL-6 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-6 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-6 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 34.58 93.20 396.79 34.39 95.73 379.80
Standard deviation 2.04 4.50 19.96 1.75 4.79 18.61
CV (%) 5.90 4.83 5.03 5.09 5.00 4.90

Recovery

The recovery of Rat IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-111 105
EDTA plasma (n=8) 95-109 102
Cell culture media (n=8) 89-95 92

Linearity

Samples were spiked with high concentrations of Rat IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P20607
SynonymsIL6, BSF2, HGF, HSF, IFNB2, CDF
Research AreaCancer, Metabolism, Immunology, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. ACS Applied Materials & Interfaces (2021) IF: 9.2289
    Self-Assembled pH-Sensitive Polymeric Nanoparticles for the Inflammation-Targeted Delivery of Cu/Zn-Superoxide Dismutase

    DOI: 10.1021/acsami.1c03589

    PMID: 33764751

    		 Sample: Plasma
  2. British journal of pharmacology (2018) IF: 6.81
    High-fructose corn syrup consumption in adolescent rats causes bipolar-like behavioural phenotype with hyperexcitability in hippocampal CA3-CA1 synapses

    DOI: 10.1111/bph.14500

    PMID: 30221753

    		 Sample: Serum
  3. Oxidative medicine and cellular longevity (2020) IF: 5.076
    Neuregulin-1 β Protects the Rat Diaphragm during Sepsis against Oxidative Stress and Inflammation by Activating the PI3K/Akt Pathway

    DOI: 10.1155/2020/1720961

    PMID: 32765805

    		 Sample: Serum,Tissue homogenate
  4. Translational Stroke Research (2020) IF: 5.78
    AAV/BBB-Mediated Gene Transfer of CHIP Attenuates Brain Injury Following Experimental Intracerebral Hemorrhage

    DOI: 10.1007/s12975-019-00715-w

    PMID: 31325153

    		 Sample: Plasma
  5. International journal of biological macromolecules (2020) IF: 5.162
    Anti-inflammatory effect of epidermal growth factor conjugated silk fibroin immobilized polyurethane ameliorates diabetic burn wound healing

    DOI: 10.1016/j.ijbiomac.2019.09.219

    PMID: 31647938

    		 Sample: Serum,Tissue homogenate
  6. development (2019) IF: 5.413
    Effects of Tarantula cubensis alcoholic extract and Nerium oleander distillate on experimentally induced colon cancer
    Sample: Serum
  7. IF: 5.57
    Delivery of siRNA using folate receptor-targeted pH-sensitive polymeric nanoparticles for rheumatoid arthritis therapy
    Sample: Plasma
  8. Nanomedicine-Nanotechnology Biology and Medicine (2019) IF: 5.57
    Delivery of siRNA using folate receptor-targeted pH-sensitive polymeric nanoparticles for rheumatoid arthritis therapy

    DOI: 10.1016/j.nano.2019.102017

    PMID: 31128293

    		 Sample: Plasma
  9. The FASEB Journal (2018) IF: 5.595
    Hypothermic oxygenated perfusion (HOPE) attenuates ischemia/reperfusion injury in the liver through inhibition of the TXNIP/NLRP3 inflammasome pathway in a rat model of donation after cardiac death.

    DOI: 10.1096/fj.201800028RR

    PMID: 29870680

    		 Sample: Tissue homogenate
  10. Cellular Physiology and Biochemistry (2017) IF: 5.104
    Bifidobacterium Infantis Ameliorates Chemotherapy-Induced Intestinal Mucositis Via Regulating T Cell Immunity In Colorectal Cancer Rats.

    DOI: 10.1159/000480005

    PMID: 28848081

    		 Sample: Serum
  11. Cellular Physiology and Biochemistry (2016) IF: 5.104
    Hyperbaric Oxygen Intervention Modulates Early Brain Injury After Experimental Subarachnoid Hemorrhage In Rats: Possible Involvement Of TLR4/NF-κB-Mediated Signaling Pathway.

    DOI: 10.1159/000445586

    PMID: 27197977

    		 Sample: Tissue homogenate
  12. Journal of Molecular and Cellular Cardiology (2016) IF: 5.68
    Cinnamaldehyde Ameliorates LPS-Induced Cardiac Dysfunction Via TLR4-NOX4 Pathway: The Regulation Of Autophagy And ROS Production.

    DOI: 10.1016/j.yjmcc.2016.10.017

    PMID: 27838370

    		 Sample: Serum
  13. European Journal of Pharmaceutics and Biopharmaceutics (2020) IF: 4.604
    Study of double-targeting nanoparticles loaded with MCL-1 siRNA and dexamethasone for adjuvant-induced arthritis therapy

    DOI: 10.1016/j.ejpb.2020.07.009

    PMID: 32681961

    		 Sample: Serum
  14. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats

    DOI: 10.1016/j.biopha.2020.110400

    PMID: 32570115

    		 Sample: Serum
  15. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Soybean isoflavones prevent atrazine-induced neurodegenerative damage by inducing autophagy

    DOI: 10.1016/j.ecoenv.2019.110065

    PMID: 31869719

    		 Sample: Tissue homogenate
  16. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Tetramethylpyrazine attenuates endotoxin-induced retinal inflammation by inhibiting microglial activation via the TLR4/NF-κB signalling pathway

    DOI: 10.1016/j.biopha.2020.110273

    PMID: 32460188

    		 Sample: Cell culture supernatant
  17. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Berberine regulates fecal metabolites to ameliorate 5-fluorouracil induced intestinal mucositis through modulating gut microbiota

    DOI: 10.1016/j.biopha.2020.109829

    PMID: 31958765

    		 Sample: Tissue homogenate
  18. Frontiers in Pharmacology (2020) IF: 4.225
    Sodium Tanshinone II Sulfonate A Ameliorates Hypoxia-Induced Pulmonary Hypertension

    DOI: 10.3389/fphar.2020.00687

    PMID: 32508639

    		 Sample: Tissue homogenate
  19. Molecular Pharmaceutics (2019) IF: 4.321
    Accelerated Blood Clearance of Nanoemulsions Modified with PEG-Cholesterol and PEG-Phospholipid Derivatives in Rats: The Effect of PEG-Lipid Linkages and PEG Molecular Weights

    DOI: 10.1021/acs.molpharmaceut.9b00770

    PMID: 31860321

    		 Sample: Serum
  20. International Journal of Biological Sciences (2019) IF: 4.858
    Hydrogen sulfide treatment alleviated ventilator-induced lung injury through regulation of autophagy and endoplasmic reticulum stress

    DOI: 10.7150/ijbs.38315

    PMID: 31853224

    		 Sample: Serum,Bronchoalveolar Lavage Fluid,Tissue homogena
  21. Journal of cellular biochemistry (2019) IF: 4.237
    Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study

    DOI: 10.1002/jcb.28976

    PMID: 31099131

    		 Sample: Tissue homogenate
  22. Neurobiology of aging (2019) IF: 4.454
    CIP2A-promoted astrogliosis induces AD-like synaptic degeneration and cognitive deficits

    DOI: 10.1016/j.neurobiolaging.2018.11.023

    PMID: 30594047

    		 Sample: Tissue homogenate,Cell culture medium
  23. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2019) IF: 4.328
    Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    		 Sample: Serum
  24. Oxidative Medicine and Cellular Longevity (2018) IF: 4.936
    Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways.

    DOI: 10.1155/2018/9647809

    PMID: 29977458

    		 Sample: Cells
  25. European Journal of Pharmaceutics and Biopharmaceutics (2018) IF: 4.491
    Dual-functional lipid polymeric hybrid pH-responsive nanoparticles decorated with cell penetrating peptide and folate for therapy against rheumatoid arthritis.

    DOI: 10.1016/j.ejpb.2018.06.020

    PMID: 29928978

    		 Sample: Plasma
  26. Scientific reports (2017) IF: 4.259
    Effect Of Qianghuo Erhuang Decoction On T Regulatory And T Helper 17 Cells In Treatment Of Adjuvant-Induced Arthritis In Rats.

    DOI: 10.1038/s41598-017-17566-w

    PMID: 29222448

    		 Sample: Serum
  27. Oxidative Medicine and Cellular Longevity (2017) IF: 4.593
    Expression And RoleOf The Calcium-Sensing Receptor In Rat Peripheral Blood Polymorphonuclear Neutrophils.

    DOI: 10.1155/2017/3869561

    PMID: 29081886

    		 Sample: Cell culture supernatant
  28. International Journal of Nanomedicine (2017) IF: 4.3
    Multifunctional Folate Receptor-targeting And pH-responsive Nanocarriers Loaded With Methotrexate For Treatment Of Rheumatoid Arthritis.

    DOI: 10.2147/IJN.S140992

    PMID: 28932117

    		 Sample: Serum
  29. Scientific Reports (2017) IF: 4.259
    Hydrogen Sulfide Ameliorates Chronic Renal Failure In Rats By Inhibiting Apoptosis And Inflammation Through ROS/MAPK And NF-κB Signaling Pathways.

    DOI: 10.1038/s41598-017-00557-2

    PMID: 28352125

    		 Sample: Tissue homogenate
  30. Oxidative Medicine & Cellular Longevity (2015) IF: 4.593
    Polydatin Alleviates Small Intestine Injury During Hemorrhagic Shock As A Sirt1 Activator.

    DOI: 10.1155/2015/965961

    PMID: 26301045

    		 Sample: Serum
  31. International Immunopharmacology (2020) IF: 3.943
    Inhibition of macrophage migration inhibitory factor prevents thyroid dysfunction in pregnant rats with acute pancreatitis

    DOI: 10.1016/j.intimp.2020.106771

    PMID: 32683302

    		 Sample: Serum
  32. Immunology letters (2020) IF: 3.276
    Protective effect of Ganoderic acid A on adjuvant-induced arthritis

    DOI: 10.1016/j.imlet.2020.06.010

    PMID: 32565114

    		 Sample: Serum
  33. European Journal of Pharmacology (2020) IF: 3.263
    iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction

    DOI: 10.1016/j.yexcr.2020.112086

    PMID: 32416091

    		 Sample: Serum,Cell sample
  34. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance

    DOI: 10.1016/j.etap.2020.103506

    PMID: 33002592

    		 Sample: Serum
  35. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Octreotide alleviates pancreatic damage caused by paraquat in rats by reducing inflammatory responses and oxidative stress

    DOI: 10.1016/j.etap.2020.103456

    PMID: 32673753

    		 Sample: Serum,Tissue homogenate
  36. Experimental Biology and Medicine (2020) IF: 3.139
    H 2 S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty

    DOI: 10.1177/1535370220961038

    PMID: 32996350

    		 Sample: Plasma
  37. European review for medical and pharmacological sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (miR-2) and miR-101 on sepsis-induced myocardial injury in rats

    DOI: 10.26355/eurrev_202006_21528
    Sample: Serum
  38. Environmental Science and Pollution Research (2020) IF: 3.056
    Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and collagen/TGF-β1 pathway

    DOI: 10.1007/s11356-020-10042-5

    PMID: 32671702

    		 Sample: Serum
  39. Cell Cycle (2020) IF: 3.699
    Knockdown of circular RNA circMAT2B reduces oxygen-glucose deprivation-induced inflammatory injury in H9c2 cells through up-regulating miR-133

    DOI: 10.1080/15384101.2020.1814025

    PMID: 32897801

    		 Sample: Cell culture supernatant
  40. Mediators of Inflammation (2020) IF: 3.758
    Paeonol Attenuates Methotrexate-Induced Cardiac Toxicity in Rats by Inhibiting Oxidative Stress and Suppressing TLR4-Induced NF- κ B Inflammatory Pathway

    DOI: 10.1155/2020/8641026

    PMID: 32104151

    		 Sample: Soil,Water,Oil,Plant?Tissue,Plant?Fluid,Antibody,F
  41. International Immunopharmacology (2020) IF: 3.943
    Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats

    DOI: 10.1016/j.intimp.2020.106582

    PMID: 32438076

    		 Sample: Plasma
  42. Obesity (2020) IF: 3.742
    Liraglutide Lowers Body Weight Set Point in DIO Rats and its Relationship with Hypothalamic Microglia Activation

    DOI: 10.1002/oby.22666

    PMID: 31773909

    		 Sample: Cell culture supernatant
  43. Inflammation (2020) IF: 3.212
    Nobiletin Protects from Renal Ischemia-Reperfusion Injury in Rats by Suppressing Inflammatory Cytokines and Regulating iNOS-eNOS Expressions

    DOI: 10.1007/s10753-019-01123-w

    PMID: 31705353

    		 Sample: Serum
  44. Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606
    Nobiletin attenuates acetaminophen-induced hepatorenal toxicity in rats

    DOI: 10.1002/jbt.22427

    PMID: 31777137

    		 Sample: Serum,Tissue homogenate
  45. International Immunopharmacology (2020) IF: 3.943
    The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats

    DOI: 10.1016/j.intimp.2020.106615

    PMID: 32447219

    		 Sample: Plasma
  46. International Immunopharmacology (2020) IF: 3.943
    The ameliorating effect of cannabinoid type 2 receptor activation on brain, lung, liver and heart damage in cecal ligation and puncture-induced sepsis model in rats

    DOI: 10.1016/j.intimp.2019.105978

    PMID: 31767546

    		 Sample: Tissue homogenate
  47. Biology of Reproduction (2019) IF: 3.322
    Changes in alpha-7 nicotinic acetylcholine receptor and macrophage polarization state participate in the regulation of cervical remodeling in pregnant rats?

    DOI: 10.1093/biolre/ioz133

    PMID: 31342065

    		 Sample: Tissue homogenate
  48. European Journal of Pharmacology (2019) IF: 3.263
    Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172786

    PMID: 31712060

    		 Sample: Serum
  49. Life Sciences (2019) IF: 3.647
    Inhibition of JAK-STAT and NF-κB signalling systems could be a novel therapeutic target against insulin resistance and type 2 diabetes

    DOI: 10.1016/j.lfs.2019.117045

    PMID: 31730866

    		 Sample: Serum
  50. Neuroscience (2018) IF: 3.056
    Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest

    DOI: 10.1016/j.neuroscience.2018.09.041

    PMID: 30300703

    		 Sample: Serum
  51. Inflammopharmacology (2018) IF: 3.304
    SB203580 attenuates acute lung injury and inflammation in rats with acute pancreatitis in pregnancy

    DOI: 10.1007/s10787-018-0522-9

    PMID: 30094758

    		 Sample: Tissue homogenate
  52. International Journal of Neuropsychopharmacology (2018) IF: 3.981
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    		 Sample: Plasma,Tissue homogenate
  53. Frontiers in pharmacology (2018) IF: 3.831
    PSTPIP2 inhibits the inflammatory response and proliferation of fibroblast-like synoviocytes in vitro

    DOI: 10.3389/fphar.2018.01432

    PMID: 30564127

    		 Sample: Serum,Cell culture supernatant
  54. Journal of cellular physiology (2019) IF: 3.923
    Long noncoding RNA MEG3 regulates rheumatoid arthritis by targeting NLRC5

    DOI: 10.1002/jcp.28126

    PMID: 30644097

    		 Sample: Serum
  55. Molecules (2019) IF: 3.098
    Plant-Derived Oleanolic Acid (OA) Ameliorates Risk Factors of Cardiovascular Diseases in a Diet-Induced Pre-Diabetic Rat Model: Effects on Selected Cardiovascular Risk Factors

    DOI: 10.3390/molecules24020340

    PMID: 30669379

    		 Sample: Plasma,Tissue homogenate
  56. Journal of Trace Elements in Medicine and Biology (2019) IF: 3.755
    Rutin abrogates manganese - induced striatal and hippocampal toxicity via inhibition of iron depletion, oxidative stress, inflammation and suppressing the NF-κB signaling pathway

    DOI: 10.1016/j.jtemb.2019.01.014

    PMID: 30910212

    		 Sample: Tissue homogenate
  57. Ecotoxicology and environmental safety (2019) IF: 3.974
    Cold stress provokes lung injury in rats co-exposed to fine particulate matter and lipopolysaccharide

    DOI: 10.1016/j.ecoenv.2018.10.064

    PMID: 30384172

    		 Sample: Tissue homogenate
  58. Journal of Cellular Physiology (2019) IF: 3.923
    C1q/TNF-related protein-9 promotes macrophage polarization and improves cardiac dysfunction after myocardial infarction

    DOI: 10.1002/jcp.28513

    PMID: 30953351

    		 Sample: Tissue homogenate
  59. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  60. Inflammopharmacology (2018) IF: 3.304
    SB203580 attenuates acute lung injury and inflammation in rats with acute pancreatitis in pregnancy

    DOI: 10.1007/s10787-018-0522-9

    PMID: 30094758

    		 Sample: Serum
  61. International Journal of Neuropsychopharmacology IF: 3.98
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    		 Sample: Serum, Tissue homogenate
  62. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    		 Sample: Serum, Tissue homogenate
  63. Phytomedicine (2018) IF: 3.61
    Epigallocatechin gallate attenuates mitochondrial DNA-induced inflammatory damage in the development of ventilator-induced lung injury.

    DOI: 10.1016/j.phymed.2018.05.017

    PMID: 30195870

    		 Sample: Tissue homogenate
  64. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

    DOI: 10.1016/j.biopha.2018.07.081

    PMID: 30119204

    		 Sample: Serum
  65. Acta Cirurgica Brasileira (2018) IF: 3.0895
    Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation.

    DOI: 10.1590/s0102-865020180030000006

    PMID: 29668774

    		 Sample: Serum
  66. Molecular Immunology (2018) IF: 3.236
    The Protective Effect Of Dexmedetomidine On LPS-Induced Acute Lung Injury Through The HMGB1-Mediated TLR4/NF-κB And PI3K/Akt/mTOR Pathways.

    DOI: 10.1016/j.molimm.2017.12.008

    PMID: 29241031

    		 Sample: Bronchoalveolar Lavage Fluid,Serum,Tissue homogena
  67. Molecular Immunology (2017) IF: 3.236
    NLRC5 Promotes Cell Proliferation Via Regulating The NF-κB Signaling Pathway In Rheumatoid Arthritis.

    DOI: 10.1016/j.molimm.2017.08.024

    PMID: 28865311

    		 Sample: Tissue homogenate,Cell lysate,Serum
  68. Phytotherapy Research (2017) IF: 3.092
    Antiinflammation Effects And Mechanisms Study Of Geniposide On Rats With Collagen-Induced Arthritis.

    DOI: 10.1002/ptr.5775

    PMID: 28127805

    		 Sample: Cell culture supernatant
  69. Basic & Clinical Pharmacology & Toxicology (2016) IF: 3.176
    Small Molecule Inhibiting NF-κB Ameliorates Oxidative Stress And Suppresses Renal Inflammation In Early Stage Of Alloxan-Induced Diabetic Nephropathy In Rat.

    DOI: 10.1111/bcpt.12718

    PMID: 27888584

    		 Sample: Tissue homogenate
  70. Physiology & Behavior (2020) IF: 2.826
    Inhibition of the fatty acid amide hydrolase changes behaviors and brain catecholamines in a sex-specific manner in rats exposed to chronic unpredictable stress

    DOI: 10.1016/j.physbeh.2020.113174

    PMID: 32966816

    		 Sample: Tissue homogenate
  71. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2020) IF: 2.842
    Activation of Nrf2 Signaling by Apelin Attenuates Renal Ischemia Reperfusion Injury in Diabetic Rats

    DOI: 10.2147/DMSO.S246743

    PMID: 32606875

    		 Sample: Serum
  72. Molecular Medicine Reports (2020) IF: 2.1
    Pretreatment with the ALDH2 activator Alda-1 protects rat livers from ischemia/reperfusion injury by inducing autophagy

    DOI: 10.3892/mmr.2020.11312

    PMID: 32705206

    		 Sample: Serum
  73. Journal of the Science of Food and Agriculture (2020) IF: 2.614
    Tiliacora triandra extract and its major constituent attenuates diabetic kidney and testicular impairment by modulating redox imbalance and pro-inflammatory responses in rats

    DOI: 10.1002/jsfa.10779

    PMID: 32875596

    		 Sample: Tissue homogenate
  74. Immunological Investigations (2020) IF: 2.511
    Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase-1/IL-1β Signaling Pathway Inhibition

    DOI: 10.1080/08820139.2020.1791178

    PMID: 32674625

    		 Sample: Cell culture supernatant
  75. Fundamental & Clinical Pharmacology (2020) IF: 2.754
    Ameliorative effect of Ozagrel, a thromboxane A2 synthase inhibitor, in hyperhomocysteinemia-induced experimental vascular cognitive impairment and dementia

    DOI: 10.1111/fcp.12610

    PMID: 33020931

    		 Sample: Tissue homogenate
  76. Journal of Ovarian Research (2020) IF: 2.989
    The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats

    DOI: 10.1186/s13048-020-00650-7

    PMID: 32386521

    		 Sample: Serum
  77. Digestive Diseases and Sciences (2020) IF: 2.751
    Apocynin Attenuates Acute Kidney Injury and Inflammation in Rats with Acute Hypertriglyceridemic Pancreatitis

    DOI: 10.1007/s10620-019-05892-0

    PMID: 31617131

    		 Sample: Serum
  78. Pharmaceutical Biology (2020) IF: 2.971
    Protective effect of apigenin magnesium complex on H 2 O 2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells

    DOI: 10.1080/13880209.2020.1772840

    PMID: 32544362

    		 Sample: Cell culture supernatant
  79. PloS one (2020) IF: 2.74
    Associations of inflammatory markers with impaired left ventricular diastolic and systolic function in collagen-induced arthritis

    DOI: 10.1371/journal.pone.0230657

    PMID: 32208438

    		 Sample: Serum
  80. Future Microbiology (2020) IF: 2.907
    The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

    DOI: 10.2217/fmb-2020-0005

    PMID: 32250184

    		 Sample: Serum
  81. Cancer chemotherapy and pharmacology (2020) IF: 2.967
    The gut microbiota attenuates muscle wasting by regulating energy metabolism in chemotherapy-induced malnutrition rats

    DOI: 10.1007/s00280-020-04060-w

    PMID: 32415349

    		 Sample: Serum,Tissue homogenate,Feces
  82. Experimental and Clinical Endocrinology & Diabetes (2019) IF: 2.058
    Hyperuricemia Inhibition Protects SD Rats Against Fructose-Induced Obesity Hypertension Via Modulation of Inflammation and Renin-Angiotensin System in Adipose Tissue

    DOI: 10.1055/a-1023-6710

    PMID: 31683330

    		 Sample: Serum
  83. Canadian journal of physiology and pharmacology (2018) IF: 2.21
    Histopathological and biochemical changes in the development of nonalcoholic fatty liver disease induced by high-sucrose diet at different times

    DOI: 10.1139/cjpp-2018-0353

    PMID: 30388378

    		 Sample: Serum
  84. Bioscience reports (2018) IF: 2.899
    Shenqi Fuzheng Injection impairs bile duct ligation-induced cholestatic liver injury in vivo

    DOI: 10.1042/BSR20180787

    PMID: 30610157

    		 Sample: Tissue homogenate
  85. Journal of Neuroimmunology (2019) IF: 2.655
    The role of UCH-L1, MMP-9, and GFAP as peripheral markers of different susceptibility to seizure development in a preclinical model of epilepsy

    DOI: 10.1016/j.jneuroim.2019.03.018

    PMID: 30952062

    		 Sample: Plasma
  86. American Journal of Reproductive Immunology (2019) IF: 2.745
    Nisin Reduces Uterine Inflammation in Rats by Modulating Concentrations of Pro- And Anti-Inflammatory Cytokines

    DOI: 10.1111/aji.13096

    PMID: 30681748

    		 Sample: Serum
  87. Bioscience reports (2018) IF: 2.899
    Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats

    DOI: 10.1042/BSR20180892

    PMID: 30232232

    		 Sample: Tissue homogenate
  88. Journal of cellular biochemistry (2019) IF: 2.959
    Exosomes derived from miR-146a-modified adipose- derived stem cells attenuate acute myocardial infarction- induced myocardial damage via downregulation of early growth response factor 1

    DOI: 10.1002/jcb.27731

    PMID: 30362610

    		 Sample: Serum,Cell culture supernatant
  89. Journal of cellular biochemistry (2019) IF: 2.959
    MAPKs and NF-κB-mediated acrylamide-induced neuropathy in rat striatum and human neuroblastoma cells SY5Y

    DOI: 10.1002/jcb.27671

    PMID: 30368882

    		 Sample: Tissue homogenate
  90. Autoimmunity (2019) IF: 2.648
    The changes in immune cell concentration during the progression of pre-diabetes to type 2 diabetes in a high-fat high-carbohydrate diet-induced pre-diabetic rat model

    DOI: 10.1080/08916934.2019.1575820

    PMID: 30776930

    		 Sample: Plasma
  91. Neurochemical research (2019) IF: 2.772
    Interleukin-1 Receptor Associated Kinase 1 Mediates the Maintenance of Neuropathic Pain after Chronic Constriction Injury in Rats

    DOI: 10.1007/s11064-019-02767-8

    PMID: 30859436

    		 Sample: Tissue homogenate
  92. ACS omega (2020) IF: 2.87
    Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies

    DOI: 10.1021/acsomega.0c01670

    PMID: 32803042

    		 Sample: Serum
  93. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    		 Sample: Plasma,Tissue homogenate
  94. Inflammation (2018) IF: 2.884
    Protective Effect of Apigenin on Acrylonitrile-Induced Inflammation and Apoptosis in Testicular Cells via the NF-κB Pathway in Rats.

    DOI: 10.1007/s10753-018-0791-x

    PMID: 29687413

    		 Sample: Plasma
  95. Neuroscience Letters (2018) IF: 2.159
    Rutin Attenuates Neurobehavioral Deficits, Oxidative Stress, Neuro-Inflammation and Apoptosis in Fluoride Treated Rats.

    DOI: 10.1016/j.neulet.2018.06.023

    PMID: 29908257

    		 Sample: Tissue homogenate
  96. Cardiovascular Toxicology (2018) IF: 2.712
    Catechin Attenuates Coronary Heart Disease In A Rat Model By Inhibiting Inflammation.

    DOI: 10.1007/s12012-018-9449-z

    PMID: 29464499
  97. Gene (2018) IF: 2.415
    MicroRNA-24 regulates vascular remodeling via inhibiting PDGF-BB pathway in diabetic rat model.

    DOI: 10.1016/j.gene.2018.03.056

    PMID: 29559348
  98. Biochemical And Biophysical Research Communications (2017) IF: 2.466
    Umbilical Cord-Derived Mesenchymal Stem Cells Alleviated Inflammation And Inhibited Apoptosis In Interstitial Cystitis Via AKT/mTOR Signaling Pathway.

    DOI: 10.1016/j.bbrc.2017.11.072

    PMID: 29137981

    		 Sample: Tissue homogenate
  99. Biochemical And Biophysical Research Communications (2017) IF: 2.466
    Edaravone Attenuates Lipopolysaccharide-induced Acute Respiratory Distress Syndrome Associated Early Pulmonary Fibrosis Via Inhibition Of Oxidative Stress And Transforming Growth Factor-β1/Smad3 Signaling.

    DOI: 10.1016/j.bbrc.2017.10.165

    PMID: 29102631

    		 Sample: Serum
  100. Inflammation (2017) IF: 2.955
    MeCP2 Regulates PTCH1 Expression Through DNA Methylation In Rheumatoid Arthritis.

    DOI: 10.1007/s10753-017-0591-8

    PMID: 28573530

    		 Sample: Cell culture supernatant
  101. Biomedicine & Pharmacotherapy (2017) IF: 2.759
    Anti-Steatotic And Anti-Inflammatory Effects Of Hovenia Dulcis Thunb. Extracts In Chronic Alcohol-Fed Rats.

    DOI: 10.1016/j.biopha.2017.03.077

    PMID: 28380415

    		 Sample: Serum
  102. International Journal of Molecular Medicine (2017) IF: 2.341
    Sirt1 Exerts Neuroprotective Effects By Attenuating Cerebral Ischemia/Reperfusion-Induced Injury Via Targeting P53/Microrna-22.

    DOI: 10.3892/ijmm.2016.2806

    PMID: 27878231

    		 Sample: Tissue homogenate
  103. BMC Complementary and Alternative Medicine (2015) IF: 2.288
    Edible Bird’S Nest Attenuates High Fat Diet-Induced Oxidative Stress And Inflammation Via Regulation Of Hepatic Antioxidant And Inflammatory Genes.

    DOI: 10.1186/s12906-015-0843-9

    PMID: 26341858

    		 Sample: Serum
  104. Journal of Surgical Research (2013) IF: 2.187
    Dobutamine-Mediated Heme Oxygenase-1 Induction Via PI3K And p38 MAPK Inhibits High Mobility Group Box 1 Protein Release And Attenuates Rat Myocardial Ischemia/Reperfusion Injury In Vivo.

    DOI: 10.1016/j.jss.2013.02.051

    PMID: 23531454

    		 Sample: Tissue homogenate
  105. Journal of Ethnopharmacology (2017) IF: 2.981
    Renoprotective And Antioxidative Effects Of Methanolic Paederia Foetida Leaf Extract On Experimental Diabetic Nephropathy In Rats.

    DOI: 10.1016/j.jep.2017.01.035

    PMID: 28111217

    		 Sample: Serum,Tissue homogenate
  106. European Journal of Pharmacology (2016) IF: 2.896
    The Protective Role Of Neuregulin-1: A Potential Therapy For Sepsis-Induced Cardiomyopathy.

    DOI: 10.1016/j.ejphar.2016.06.042

    PMID: 27346832

    		 Sample: Serum
  107. Plos One (2015) IF: 2.806
    Transplantation Of Induced Pluripotent Stem Cells Alleviates Cerebral Inflammation And Neural Damage In Hemorrhagic Stroke.

    DOI: 10.1371/journal.pone.0129881

    PMID: 26086994

    		 Sample: Tissue homogenate
  108. Microbial Pathogenesis (2016) IF: 2.009
    Candida Glabrata Induced Infection Of Rat Tracheal Epithelial Cells Is Mediated By TLR-2 Induced Activation Of NF-κB.

    DOI: 10.1016/j.micpath.2015.10.009

    PMID: 26477714

    		 Sample: Cell lysate
  109. Journal of Ethnopharmacology (2016) IF: 2.981
    Venenum Bufonis Induces Rat Neuroinflammation By Activiating NF-κB Pathway And Attenuation Of BDNF.

    DOI: 10.1016/j.jep.2016.03.049

    PMID: 27013094

    		 Sample: Tissue homogenate
  110. Journal of Biomedical Science (2015) IF: 2.799
    High Fat Diet-Induced Inflammation And Oxidative Stress Are Attenuated By N-Acetylneuraminic Acid In Rats.

    DOI: 10.1186/s12929-015-0211-6

    PMID: 26498218

    		 Sample: Serum
  111. International Immunopharmacology (2015) IF: 2.956
    Anti-Inflammatory Effects And Pharmacokinetics Study Of Geniposide On Rats With Adjuvant Arthritis.

    DOI: 10.1016/j.intimp.2014.11.017

    PMID: 25434608

    		 Sample: Cell culture supernatant
  112. International Immunopharmacology (2014) IF: 2.956
    Effects And Mechanisms Of Geniposide On Rats With Adjuvant Arthritis.

    DOI: 10.1016/j.intimp.2014.02.021

    PMID: 24583144

    		 Sample: Cell lysate
  113. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research (2020) IF: 1.918
    Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

    DOI: 10.12659/MSM.919054

    PMID: 31992687

    		 Sample: Plasma
  114. Asian Journal of Surgery (2020) IF: 1.838
    Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid

    DOI: 10.1016/j.asjsur.2019.07.001

    PMID: 31345657

    		 Sample: Tissue homogenate
  115. in vivo (2019) IF: 1.541
    Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

    DOI: 10.21873/invivo.11624

    PMID: 31471392

    		 Sample: Bronchoalveolar Lavage Fluid
  116. Molecular Biology Reports (2019) IF: 1.402
    Protective effects of Anthocleista djalonensis A. Chev root extracts against induced testicular inflammation and impaired spermatogenesis in adult rats

    DOI: 10.1007/s11033-019-05033-w

    PMID: 31428909

    		 Sample: Serum,Tissue homogenate
  117. Annals of Surgical Treatment and Research (2019) IF: 1.367
    Examination of Protective and Therapeutic Effects of Ruscogenin on Cerulein-Induced Experimental Acute Pancreatitis in Rats

    DOI: 10.4174/astr.2019.97.6.271

    PMID: 31824881

    		 Sample: Serum,Tissue homogenate
  118. Biological and Pharmaceutical Bulletin (2018) IF: 1.54
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    		 Sample: Plasma
  119. Experimental and therapeutic medicine (2018) IF: 1.41
    Protective effect of dexpanthenol against cisplatin-induced hepatotoxicity

    DOI: 10.3892/etm.2018.6683

    PMID: 30402149

    		 Sample: Tissue homogenate
  120. Biotechnology & Biotechnological Equipment (2018) IF: 1.227
    Effect of polysaccharides from Vitis vinifera L. on NF-κB/IκB-α signal pathway and inflammatory factors in Alzheimer's model rats.

    DOI: 10.1080/13102818.2018.1464948
    Sample: Serum
  121. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Bletilla striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAAinduced cirrhotic rats.

    DOI: 10.3892/etm.2018.6430

    PMID: 30186392

    		 Sample: Serum,Tissue homogenate
  122. Iranian Journal of Basic Medical Sciences (2018) IF: 1.424
    The Protective Effects Of Naringin Against 5-Fluorouracil-Induced Hepatotoxicity And Nephrotoxicity In Rats.

    DOI: 10.22038/IJBMS.2018.27510.6714

    PMID: 29796225
  123. Molecular Medicine Reports (2014) IF: 1.692
    IsoproterenolMediated Heme Oxygenase1 Induction Inhibits High Mobility Group Box 1 Protein Release And Protects Against Rat Myocardial Ischemia/Reperfusion Injury In Vivo.

    DOI: 10.3892/mmr.2014.2026

    PMID: 24604346

    		 Sample: Tissue homogenate
  124. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Astragalus Polysaccharides Combined With Ibuprofen Exhibit A Therapeutic Effect On Septic Rats Via An Anti-Inflammatory Cholinergic Pathway.

    DOI: 10.3892/etm.2017.4865

    PMID: 28912862

    		 Sample: Plasma
  125. Experimental and Therapeutic Medicine (2018) IF: 1.261
    Gambogic Acid Exerts Cardioprotective Effects In A Rat Model Of Acute Myocardial Infarction Through Inhibition Of Inflammation, iNOS And NFκB/p38 Pathway.

    DOI: 10.3892/etm.2017.5599

    PMID: 29434760

    		 Sample: Serum
  126. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Tocotrienol Alleviates Inflammation And Oxidative Stress In A Rat Model Of Spinal Cord Injury Via Suppression Of Transforming Growth Factor-β.

    DOI: 10.3892/etm.2017.4505

    PMID: 28672950

    		 Sample: Serum
  127. Medical Science Monitor (2017) IF: 1.585
    Epigallocatechin Gallate Attenuates Hip Fracture-Induced Acute Lung Injury By Limiting Mitochondrial Dna (Mtdna) Release.

    DOI: 10.12659/MSM.902477

    PMID: 28698540

    		 Sample: Bronchoalveolar Lavage Fluid
  128. Toxicology Research (2017) IF: 1.969
    Nano NIO Induced Liver Toxicity Via Activating The NF-κB Signaling Pathway In Rats.

    DOI: 10.1039/c6tx00444j

    PMID: 30090495

    		 Sample: Tissue homogenate
  129. Brazilian Journal of Medical and Biological Research (2017) IF: 1.578
    Angiotensin-(1-7) Relieved Renal Injury Induced By Chronic Intermittent Hypoxia In Rats By Reducing Inflammation, Oxidative Stress And Fibrosis.

    DOI: 10.1590/1414-431X20165594

    PMID: 28076452

    		 Sample: Plasma
  130. Molecular Medicine Reports (2015) IF: 1.692
    Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism.

    DOI: 10.3892/mmr.2015.4425

    PMID: 26458544

    		 Sample: Serum
  131. Immunopharmacology & Immunotoxicology (2016) IF: 1.475
    Anti-Oxidant And Anti-Inflammatory Effects Of Apigenin In A Rat Model Of Sepsis: An Immunological, Biochemical, And Histopathological Study.

    DOI: 10.3109/08923973.2016.1173058

    PMID: 27144896

    		 Sample: Serum
  132. Journal of Inflammation Research (2020)
    Pharmacological Inhibition of Galectin-3 Ameliorates Diabetes-Associated Cognitive Impairment, Oxidative Stress and Neuroinflammation in vivo and in vitro

    DOI: 10.2147/JIR.S273858

    PMID: 32982368

    		 Sample: Cell culture supernatant,Tissue homogenate
  133. Naunyn-Schmiedeberg's archives of pharmacology (2020)
    Protective effect of oleuropein on ketamine-induced cardiotoxicity in rats

    DOI: 10.1007/s00210-020-01870-w

    PMID: 32383030

    		 Sample: Tissue homogenate
  134. Journal of Family and Reproductive Health (2020)
    Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats

    DOI: 10.1177/1535370220931038

    PMID: 32493122

    		 Sample: Tissue homogenate
  135. Advances in Traditional Medicine (2020)
    Anti-inflammatory activity of Xanthium indicum on carrageenan-induced paw edema in rats

    DOI: 10.1007/s13596-020-00502-1
    Sample: Tissue homogenate
  136. Medicinal Plant (2017)
    Protection of Dicliptera chinensis Polysaccharide Granule on Acute Liver Injury of Rat Caused by D-galactosamine
    Sample: Serum,Tissue Slice
  137. Asian Journal of Research in Biochemistry (2019)
    Effect of Oral Intake of Sodium Benzoate on Serum Cholesterol and Proinflammatory Cytokine (Tumor Necrosis Factor Alpha [TNF-α] and Interleukin-6 [IL-6]) Levels in the Heart Tissue of Wistar Rats

    DOI: 10.9734/ajrb/2019/v5i230086
    Sample: Serum,Tissue homogenate
  138. Biomedical and Pharmacology Journal (2019)
    Therapeutic Intervention of Curcumin on Interleukin-6 and Oxidative Stress Induced by Paraquat Toxicity of Lung and Liver in Rats

    DOI: 10.13005/bpj/1803
    Sample: Serum
  139. New Trends in Medicine Sciences (2020)
    Role of 6-Shogaol Against Ovarian Torsion Detorsion-Induced Reproductive Organ Damage
    Sample: Tissue homogenate
  140. The Egyptian Journal of Forensic Sciences and Applied Toxicology (2020)
    ANTI-INFLAMMATORY AND ANTIOXIDANT PROPERTIES OF ECBALLIUM ELATERIUM FRUIT JUICE AGAINST CYCLOPHPSPHAMIDE INDUCED HEPATOTOXICITY IN RATS
    Sample: Plasma
  141. GSC Biological and Pharmaceutical Sciences (2019)
    Protective effects of naringin in indomethacin-induced gastric ulcer in rats

    DOI: 10.30574/gscbps.2019.8.2.0132
    Sample: Tissue homogenate
  142. Journal of Experimental Pharmacology (2020)
    Alpha-Mangostin Improves Cardiac Hypertrophy and Fibrosis and Associated Biochemical Parameters in High-Fat/High-Glucose Diet and Low-Dose Streptozotocin Injection-Induced Type 2 Diabetic Rats

    DOI: 10.2147/JEP.S233111

    PMID: 32095085

    		 Sample: Tissue homogenate
  143. International Journal of Clinical and Experimental Pathology (2020) IF: 0.252
    Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway

    PMID: 32509067

    		 Sample: Serum
  144. Borneo Journal of Pharmacy (2020)
    Level of Cytokine Interleukin-6 and Interleukin 1-β on Infectious Rat Model Treated with Etlingera elatior (Jack) RM Smith Fruit Extract as Immunomodulator

    DOI: 10.33084/bjop.v3i2.1318
    Sample: Plasma
  145. WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES (2018)
    SERUM INTERLEUKIN-6 AND GASTRIC MUCOSA EXPRESSION OF CD3 AND CD20 IN DYSTHYROID RATS

    DOI: 10.20959/wjpps20184-11252
    Sample: Serum,Tissue homogenate
  146. Pathology-Research and Practice (2018)
    miR-93-3p alleviates lipopolysaccharide-induced inflammation and apoptosis in H9c2 cardiomyocytes by inhibiting toll-like receptor 4

    DOI: 10.1016/j.prp.2018.08.024

    PMID: 30195636

    		 Sample: Cell culture supernatant
  147. Mustansiriya Medical Journal (2018)
    Evaluation of the effects of TAK-242 and GIT-27 on methotrexate-induced liver injury

    DOI: 10.4103/MJ.MJ_15_18
    Sample: Serum
  148. Synergy (2019)
    Effects of salmon calcitonin and omega-3fatty acids on selected biomarkers in experimental diabetic - osteoarthritic rats

    DOI: 10.1016/j.synres.2018.100045
    Sample: Plasma,Serum
  149. North-West University (2018)
    Studies on Garcinia mangostana Linn as a therapeutic intervention in an immune-inflammatory model of schizophrenia
    Sample: Plasma
  150. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2019)
    Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats.

    DOI: 10.2147/DMSO.S183811

    PMID: 30858714

    		 Sample: Tissue homogenate,Plasma
  151. Indonesian Journal of Tropical and Infectious Disease (2019)
    EFFECT OF AFRICAN LEAF (VERNONIA AMYGDALINA) TO IL-6 AND IL-10 LEVEL ON STAPHYLOCOCCUC AUREUS INFECTION

    DOI: 10.20473/ijtid.v7i4.9654
    Sample: Whole Blood
  152. Pathophysiology (2019)
    Pathophysiology of iron overload-induced renal injury and dysfunction: Roles of renal oxidative stress and systemic inflammatory mediators

    DOI: 10.1016/j.pathophys.2019.03.002

    PMID: 30910397

    		 Sample: Serum
  153. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.166
    Effects of methylene blue on the nitric oxide-soluble guanylate cyclase-cyclic guanylyl monophosphate pathway and cytokine levels in rats with sepsis
    Sample: Serum,Tissue homogenate
  154. bioRxiv (2018) IF: /
    Autophagy Decreases Alveolar Epithelial Cell Injury by Suppressing the NF-κB Signaling Pathway and Regulating the Release of Inflammatory Mediators.

    DOI: 10.1101/328039
    Sample: Tissue homogenate
  155. Acta Cirurgica Brasileira (2016) IF: 0.729
    Toll-Like Receptor 7 Involves The Injury In Acute Kidney Ischemia/Reperfusion Of STZ-Induced Diabetic Rats.

    DOI: 10.1590/S0102-865020160070000004

    PMID: 27487279

    		 Sample: Serum
  156. Renal Failure (2016) IF: 0.79
    Effect Of Apelin Hormone On Renal Ischemia/Reperfusion Induced Oxidative Damage In Rats.

    DOI: 10.1080/0886022X.2016.1184957

    PMID: 27197832

    		 Sample: Serum
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Reviews/Q&A

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Q

SSSubmitted [ Oct 03 2022 ]

Asked: Does this work in supernatant from harvested rat brain samples?

Reply

A

adminSubmitted [ Oct 03 2022 ]

Answered: EL-R0015 kit is suitable for the detection of rat brain tissue samples. We have verified normal rat whole brain tissue undilution and the sample measured value was 20-100pg/mL. However, there are individual differences between samples, so it is suggested that you can confirm it through pre-experiment. In addition, it is not recommended to use special lysates when collecting tissue samples. In generall, the tissue samples can be collected as follows for ELISA assays. 1) Mince the tissues to small pieces as much as you can and rinse them in ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. 2) Tissue pieces should be weighed and then homogenized in PBS (tissue weight (g): PBS volume (mL) =1:9) with a glass homogenizer on ice(It's suggested to add an appropriate amount of protease inhibitor to PBS before the homogenization). 3) To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. 4) The homogenates are then centrifuged for 5 minutes at 5000×g at 2 - 8℃, collect the supernatant to carry out the assay. To get more accurate result, it's suggested to run the assay with freshly collected samples.

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