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Cat.No.:E-EL-R2466
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Assay type | Sandwich-ELISA |
Format | 96T |
Assay time | 3.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.19 ng/mL |
Sample type &Sample volume | cell culture supernatant and tissue homogenate; 100μL |
Specificity | This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in cell culture supernatant and tissue homogenate. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat INS were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.99 | 2.68 | 6.73 | 1.00 | 2.82 | 6.90 |
Standard deviation | 0.06 | 0.14 | 0.25 | 0.07 | 0.16 | 0.35 |
CV (%) | 6.06 | 5.22 | 3.71 | 7.00 | 5.67 | 5.07 |
The recovery of Rat INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Cell culture media (n=8) | 86-98 | 92 |
Samples were spiked with high concentrations of Rat INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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DOI: 10.1039/c9tb02728a |
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PMID: 32067015 |
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DOI: 10.1039/d0tb00561d |
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PMID: 32373901 |
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DOI: 10.3390/biom8040149 |
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PMID: 30445784 |
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DOI: 10.1038/srep29582 |
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PMID: 27387420 |
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PMID: 31356906 |
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DOI: 10.1055/a-1014-2544 |
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DOI: 10.1042/BSR20180644 |
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PMID: 30291215 |
Sample: Cell culture supernatant |
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Sample: Plasma |
PMID: 31938037 |
Sample: Serum,Cell culture medium |
DOI: 10.1155/2017/4107943 |
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PMID: 29410963 |
Sample: Cell culture supernatant |
DOI: 10.1111/jfbc.13473 |
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PMID: 32964438 |
Sample: Plasma |
PMID: 32963600 |
Sample: Tissue homogenate |
DOI: 10.4149/gpb_2019025 |
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DOI: 10.1002/fsn3.988 |
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PMID: 31024722 |
Sample: Tissue homogenate,Serum |
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Sample: Cell culture supernatant |
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