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Rat INS(Insulin) ELISA Kit

Citations(39)Uniprot : P01322,P01323

  • Cat.No.:E-EL-R2466

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume cell culture supernatant and tissue homogenate; 100μL
Specificity This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in cell culture supernatant and tissue homogenate.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat INS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.99 2.68 6.73 1.00 2.82 6.90
Standard deviation 0.06 0.14 0.25 0.07 0.16 0.35
CV (%) 6.06 5.22 3.71 7.00 5.67 5.07

Recovery

The recovery of Rat INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Cell culture media (n=8) 86-98 92

Linearity

Samples were spiked with high concentrations of Rat INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01322  P01323
SynonymsIDDM2, ILPR, IRDN, MODY10
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Materials Chemistry B (2020) IF: 5.344
    A sustained zero-order release carrier for long-acting, peakless basal insulin therapy

    DOI: 10.1039/c9tb02728a

    PMID: 32067015

    		 Sample: Plasma
  2. Journal of Materials Chemistry B (2020) IF: 5.344
    A bio-inspired injectable hydrogel as a cell platform for real-time glycaemic regulation

    DOI: 10.1039/d0tb00561d

    PMID: 32373901

    		 Sample: Plasma
  3. Stem cell research & therapy (2019) IF: 5.116
    Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats

    DOI: 10.1186/s13287-019-1474-8

    PMID: 31747961

    		 Sample: Serum
  4. Cardiovascular drugs and therapy (2020) IF: 4.069
    Titin and CK2α are New Intracellular Targets in Acute Insulin Application-Associated Benefits on Electrophysiological Parameters of Left Ventricular Cardiomyocytes From Insulin-Resistant Metabolic Syndrome Rats

    DOI: 10.1007/s10557-020-06974-2

    PMID: 32377826

    		 Sample: Serum
  5. Biomolecules (2018) IF: 4.694
    Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study

    DOI: 10.3390/biom8040149

    PMID: 30445784

    		 Sample: Cell culture medium
  6. Scientific Reports (2016) IF: 4.259
    FGF21 Ameliorates The Neurocontrol Of Blood Pressure In The High Fructose-Drinking Rats.

    DOI: 10.1038/srep29582

    PMID: 27387420

    		 Sample: Serum
  7. Pflügers Archiv-European Journal of Physiology (2020) IF: 3.158
    Nitric oxide interacts with cholinoceptors to modulate insulin secretion by pancreatic β cells

    DOI: 10.1007/s00424-020-02443-9

    PMID: 32803305

    		 Sample: Cell culture supernatant
  8. Life Sciences (2019) IF: 3.647
    Inhibition of JAK-STAT and NF-κB signalling systems could be a novel therapeutic target against insulin resistance and type 2 diabetes

    DOI: 10.1016/j.lfs.2019.117045

    PMID: 31730866

    		 Sample: Serum
  9. Life sciences (2019) IF: 3.647
    Effects of noopept on cognitive functions and pubertal process in rats with diabetes

    DOI: 10.1016/j.lfs.2019.116698

    PMID: 31356906

    		 Sample: Serum,Tissue homogenate
  10. Frontiers in endocrinology (2019) IF: 3.644
    Reduced Expression of PLCXD3 Associates With Disruption of Glucose Sensing and Insulin Signaling in Pancreatic β-Cells

    DOI: 10.3389/fendo.2019.00735

    PMID: 31781030

    		 Sample: Cell culture supernatant
  11. Brain research bulletin (2019) IF: 3.44
    Role of simvastatin and insulin in memory protection in a rat model of diabetes mellitus and dementia

    DOI: 10.1016/j.brainresbull.2018.10.012

    PMID: 30395886

    		 Sample: Serum
  12. Neuroscience bulletin (2019) IF: 3.155
    Liraglutide Ameliorates Hyperhomocysteinemia-Induced Alzheimer-Like Pathology and Memory Deficits in Rats via Multi-molecular Targeting

    DOI: 10.1007/s12264-018-00336-7

    PMID: 30632006

    		 Sample: Plasma
  13. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  14. Archives of Physiology and Biochemistry (2020) IF: 2.575
    Angiotensin converting enzyme inhibitor potentiates the hypoglycaemic effect of NG-nitro-L-arginine methyl ester (L-NAME) in rats

    DOI: 10.1080/13813455.2020.1780263

    PMID: 32584611

    		 Sample: Serum
  15. Journal of Pharmacy and Pharmacology (2020) IF: 2.571
    Tropisetron improves pancreas function and increases insulin synthesis and secretion in the STZ-induced diabetic rats: involvement of UCP2/ZnT8 pathway

    DOI: 10.1111/jphp.13278

    PMID: 32349166

    		 Sample: Serum
  16. Naunyn-Schmiedeberg's Archives of Pharmacology (2020) IF: 2.05
    Possible mechanisms mediating the protective effect of cilostazol in L-arginine induced acute pancreatitis in rats: role of cGMP, cAMP, and HO-1

    DOI: 10.1007/s00210-020-01897-z

    PMID: 32424476

    		 Sample: Serum
  17. Journal of Clinical Psychopharmacology (2019) IF: 2.7
    Insulin Resistance-Related Proteins Are Overexpressed in Patients and Rats Treated With Olanzapine and Are Reverted by Pueraria in the Rat Model

    DOI: 10.1097/JCP.0000000000001028

    PMID: 30946280

    		 Sample: Plasma
  18. Experimental and Clinical Endocrinology & Diabetes (2019) IF: 2.058
    Reduced Expression of Chl1 gene Impairs Insulin Secretion by Down-Regulating the Expression of Key Molecules of β-cell Function

    DOI: 10.1055/a-1014-2544

    PMID: 31614370

    		 Sample: Cell culture supernatant
  19. International journal of environmental research and public health (2019) IF: 2.849
    The Beneficial Effects of Stingless Bee Honey from Heterotrigona itama against Metabolic Changes in Rats Fed with High-Carbohydrate and High-Fat Diet

    DOI: 10.3390/ijerph16244987

    PMID: 31817937

    		 Sample: Serum
  20. Archives of Physiology and Biochemistry (2019) IF: 2.575
    Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat

    DOI: 10.1080/13813455.2019.1668018
    Sample: Plasma
  21. International journal of molecular medicine (2019) IF: 2.784
    Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR

    DOI: 10.3892/ijmm.2018.3977

    PMID: 30431108

    		 Sample: Serum
  22. Bioscience reports (2018) IF: 2.899
    The Chinese medicine Chai Hu Li Zhong Tang protects against non-alcoholic fatty liver disease by activating AMPKα.

    DOI: 10.1042/BSR20180644

    PMID: 30291215

    		 Sample: Cell culture supernatant
  23. Canadian journal of physiology and pharmacology (2018) IF: 2.21
    The pectin-insulin patch application prevents the onset of peripheral neuropathy-like symptoms in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2018-0415

    PMID: 30326192

    		 Sample: Plasma
  24. Chinese Medicine (2020) IF: 2.96
    Protective effects of essential oil from Fructus Alpiniae zerumbet on retinal Müller gliosis via the PPAR-γ-p-CREB signaling pathway

    DOI: 10.1186/s13020-019-0283-4

    PMID: 31938037

    		 Sample: Serum,Cell culture medium
  25. BioMed Research International (2017) IF: 2.476
    Cotransplantation Of mesenchymal Stem Cells And Immature Dendritic Cells Potentiates The Blood Glucose

    DOI: 10.1155/2017/4107943

    PMID: 29410963

    		 Sample: Cell culture supernatant
  26. Journal of Food Biochemistry (2020) IF: 1.662
    Cognitive impairment by non-insulin-dependent diabetes mellitus was attenuated by dietary supplements of marble vine (Dioclea reflexa) and plantain (Musa paradisiaca) dough meals in albino rats

    DOI: 10.1111/jfbc.13473

    PMID: 32964438

    		 Sample: Plasma
  27. Experimental and Therapeutic Medicine (2020) IF: 1.785
    CGA ameliorates cognitive decline by regulating the PI3K/AKT signaling pathway and neurotransmitter systems in rats with multi-infarct dementia

    DOI: 10.3892/etm.2020.9198

    PMID: 32963600

    		 Sample: Tissue homogenate
  28. General physiology and biophysics (2019) IF: 1.07
    The potential developmental programming effect of oral curcumin on the bone health and plasma total osteocalcin of male and female rats fed a high-fructose diet during suckling and post weaning

    DOI: 10.4149/gpb_2019025

    PMID: 31411571

    		 Sample: Plasma
  29. South African Journal of Botany (2019) IF: 1.442
    Moringa oleifera Lam. prevents the development of high fructose diet-induced fatty liver

    DOI: 10.1016/j.sajb.2018.12.003
    Sample: Serum
  30. Food Science & Nutrition (2019) IF: 1.521
    Aqueous leaf extract of Clinacanthus nutans improved metabolic indices and sorbitol-related complications in type II diabetic rats (T2D)

    DOI: 10.1002/fsn3.988

    PMID: 31024722

    		 Sample: Tissue homogenate,Serum
  31. Chinese Journal of Natural Medicines (2014) IF: 1.667
    Modified Si-Miao-San Ameliorates Pancreatic B Cell Dysfunction By Inhibition Of Reactive Oxygen Species-Associated Inflammation Through Amp-Kinase Activation.

    DOI: 10.1016/S1875-5364(14)60043-1

    PMID: 24856758

    		 Sample: Cell culture supernatant
  32. Eastern Anatolian Journal of Science (2020)
    Peripheral Adropin Application Regulates Nutritional Behavior and Fat Tissue-mediated Energy Metabolism
    Sample: Serum,Tissue homogenate
  33. Pharmacognosy Journal (2020)
    Naringenin and Vanillin Mitigate Cadmium-Induced Pancreatic Injury in Rats via Inhibition of JNK and p38 MAPK Pathways

    DOI: 10.5530/pj.2020.12.107
    Sample: Serum
  34. Naunyn-schmiedeberg's Archives of Pharmacology (2020)
    Role of glucocorticoid receptor phosphorylation-mediated synaptic plasticity in anxiogenic and depressive behaviors induced by monosodium glutamate

    DOI: 10.1007/s00210-020-01845-x

    PMID: 32444989

    		 Sample: Serum
  35. Journal of dietary supplements (2019)
    High-Dose Perinatal Folic-Acid Supplementation Alters Insulin Sensitivity in Sprague-Dawley Rats and Diminishes the Expression of Adiponectin

    DOI: 10.1080/19390211.2018.1426076

    PMID: 29451831

    		 Sample: Serum,Tissue homogenate
  36. Heliyon (2019)
    Adverse effects of noise stress on glucose homeostasis and insulin resistance in Sprague-Dawley rats

    DOI: 10.1016/j.heliyon.2019.e03004

    PMID: 31890958

    		 Sample: Serum
  37. Wuhan University Journal of Natural Sciences (2019)
    Mechanism Research of Reducing Obesity-Induced Insulin Resistance in the White Adipose Tissue by Knockdown of Neuropeptide Y Expression in the Dorsomedial Hypothalamus

    DOI: 10.1007/s11859-019-1367-9
    Sample: Plasma
  38. United States Patent Application Publication (2018)
    Compounds, Compositions and Methods for Prevention or Treatment of Liver and Lipid-Related Conditions
    Sample: Plasma
  39. Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (2018)
    Effects of Moringa oleifera on Insulin Levels and Folliculogenesis in Polycystic Ovary Syndrome Model with Insulin Resistance

    DOI: 10.2174/1871522218666180426100754

    PMID: 30369967

    		 Sample: Serum
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