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Rat LH(Luteinizing Hormone) ELISA Kit

  • Cat.No.:E-EL-R0026

  • Reactivity: Rat

To Purchase E-EL-R0026

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 mIU/mL
Sensitivity 0.94 mIU/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat LH in samples. No significant cross-reactivity or interference between Rat LH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat LH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (mIU/mL) 4.70 15.40 40.80 5.00 15.10 38.80
Standard deviation 0.20 0.70 1.70 0.30 0.90 1.60
CV (%) 4.26 4.55 4.17 6.00 5.96 4.12


The recovery of Rat LH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 99
EDTA plasma (n=8) 93-105 98
Cell culture media (n=8) 90-105 98


Samples were spiked with high concentrations of Rat LH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsLutropin, Lutrophin
Research AreaNeuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

  1. Nature Communications (2021) IF: 14.919
    The Goto-Kakizaki rat is a spontaneous prototypical rodent model of polycystic ovary syndrome

    DOI: 10.1038/s41467-021-21308-y

    PMID: 33594056

    Sample: Serum
  2. Environmental Pollution (2019) IF: 6.792
    Selenium abates reproductive dysfunction via attenuation of biometal accumulation, oxido-inflammatory stress and caspase-3 activation in male rats exposed to arsenic

    DOI: 10.1016/j.envpol.2019.113079

    PMID: 31473390

    Sample: Serum
  3. Food&Function (2022) IF: 5.396
    Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

    DOI: 10.1039/d1fo01744f

    PMID: 35044398

    Sample: Serum
  4. Chemosphere (2019) IF: 5.778
    Impact of binary waterborne mixtures of nickel and zinc on hypothalamic-pituitary-testicular axis in rats

    DOI: 10.1016/j.chemosphere.2019.124501

    PMID: 31398612

    Sample: Serum
  5. Cell Death & Disease (2017) IF: 5.965
    ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.

    DOI: 10.1038/cddis.2017.494

    PMID: 29072679

    Sample: Serum
  6. Cellular Physiology and Biochemistry (2017) IF: 5.104
    Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR.

    DOI: 10.1159/000457881

    PMID: 28214901

    Sample: Serum
  7. Toxicology (2020) IF: 4.099
    Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats

    DOI: 10.1016/j.tox.2020.152556

    PMID: 32758512

    Sample: Serum,Tissue homogenate
  8. Food and Chemical Toxicology (2020) IF: 4.679
    Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

    DOI: 10.1016/j.fct.2019.110897

    PMID: 31654709

    Sample: Serum
  9. Food & Function (2020) IF: 4.171
    Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota

    DOI: 10.1039/c9fo02554e

    PMID: 32441726

    Sample: Serum
  10. Journal of Endocrinology (2016) IF: 4.706
    Reproductive Alterations In Hyperinsulinemic But Normoandrogenic MSG-Obese Female Rats.

    DOI: 10.1530/JOE-15-0453

    PMID: 26952035

    Sample: Serum
  11. Reproductive Toxicology (2020) IF: 3.121
    Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosis

    DOI: 10.1016/j.reprotox.2020.08.014

    PMID: 32911041

    Sample: Plasma
  12. Environmental Science and Pollution Research (2020) IF: 3.056
    Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats

    DOI: 10.1007/s11356-020-10324-y

    PMID: 32812151

    Sample: Serum,Tissue homogenate
  13. Environmental Science and Pollution Research (2020) IF: 3.056
    The gut-microbiota-testis axis mediated by the activation of the Nrf2 antioxidant pathway is related to prepuberal steroidogenesis disorders induced by di-(2-ethylhexyl) phthalate

    DOI: 10.1007/s11356-020-09854-2

    PMID: 32588312

    Sample: Serum,Tissue homogenate
  14. Life Sciences (2020) IF: 3.647
    Protective role of glutamine against cadmium-induced testicular dysfunction in Wistar rats: Involvement of G6PD activity

    DOI: 10.1016/j.lfs.2019.117250

    PMID: 31899225

    Sample: Serum
  15. Life sciences (2019) IF: 3.647
    Effects of noopept on cognitive functions and pubertal process in rats with diabetes

    DOI: 10.1016/j.lfs.2019.116698

    PMID: 31356906

    Sample: Serum,Tissue homogenate
  16. Cancer Cell International (2019) IF: 3.96
    Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague-Dawley rats

    DOI: 10.1186/s12935-019-0805-2

    PMID: 30992692

    Sample: Serum
  17. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    Sample: Plasma,Tissue homogenate
  18. Toxicology Letters (2019) IF: 3.499
    Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats

    DOI: 10.1016/j.toxlet.2019.10.024

    PMID: 31689472

    Sample: Serum
  19. Andrology (2018) IF: 3.259
    Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy.

    DOI: 10.1111/andr.12490

    PMID: 29654715

    Sample: Serum
  20. Andrology (2020) IF: 2.86
    Orlistat attenuates obesity-induced decline in steroidogenesis and spermatogenesis by up-regulating steroidogenic genes

    DOI: 10.1111/andr.12824

    PMID: 32438512

    Sample: Tissue homogenate
  21. International Journal of Endocrinology (2020) IF: 2.299
    Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

    DOI: 10.1155/2020/1814867

    PMID: 32148483

    Sample: Serum
  22. Toxicology Mechanisms and Methods (2020) IF: 2.295
    Cilostazol protects against cyclophosphamide-induced ovarian toxicity in female rats: role of cAMP and HO-1

    DOI: 10.1080/15376516.2020.1774829

    PMID: 32456565

    Sample: Serum
  23. BMC complementary and alternative medicine (2019) IF: 2.833
    The ameliorative effect of monotropein, astragalin, and spiraeoside on oxidative stress, endoplasmic reticulum stress, and mitochondrial signaling pathway in varicocelized rats

    DOI: 10.1186/s12906-019-2736-9

    PMID: 31771569

    Sample: Serum
  24. International journal of molecular medicine (2019) IF: 2.784
    Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR

    DOI: 10.3892/ijmm.2018.3977

    PMID: 30431108

    Sample: Serum
  25. International Journal of Molecular Medicine (2018) IF: 2.784
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    Sample: Serum
  26. Reproductive Biology and Endocrinology (2018) IF: 2.849
    GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile.

    DOI: 10.1186/s12958-018-0354-x

    PMID: 29642911

    Sample: Serum
  27. Reproductive Biology and Endocrinology (2018) IF: 2.849
    GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile.

    DOI: 10.1186/s12958-018-0354-x

    PMID: 29642911

  28. Autonomic Neuroscience-Basic & Clinical (2015) IF: 2.225
    Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats.

    DOI: 10.1016/j.autneu.2015.02.005

    PMID: 25784152

    Sample: Plasma
  29. Andrology (2017) IF: 2.427
    Naringenin Attenuates Highly Active Antiretroviral Therapy-Induced Sperm DNA Fragmentations And Testicular Toxicity In Sprague-Dawley Rats.

    DOI: 10.1111/andr.12439

    PMID: 29179260

    Sample: Serum
  30. Journal of Assisted Reproduction & Genetics (2015) IF: 2.163
    Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.

    DOI: 10.1007/s10815-015-0506-2

    PMID: 26081124

    Sample: Serum
  31. Chinese Journal of Physiology (2020) IF: 1.151
    Low-frequency electroacupuncture at acupoints guanyuan (CV4) and zhongji (CV3) lengthen ejaculatory latency and improves sexual behavior in male rats

    DOI: 10.4103/CJP.CJP_34_20

    PMID: 32859883

    Sample: Serum
  32. International Journal of Environmental Health Research (2020) IF: 1.916
    Effects of prenatal and lactational bisphenol a and/or di(2-ethylhexyl) phthalate exposure on male reproductive system

    DOI: 10.1080/09603123.2020.1805416

    PMID: 32787440

    Sample: Plasma
  33. Evidence-Based Complementary and Alternative Medicine (2020) IF: 1.813
    Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway

    DOI: 10.1155/2020/6723204

    PMID: 32184896

    Sample: Serum
  34. Journal of Clinical Research in Pediatric Endocrinology (2020) IF: 1.803
    Effects of 5-Hydroxymethylfurfural on Pubertal Development of Female Wistar Rats

    DOI: 10.4274/jcrpe.galenos.2019.2019.0080

    PMID: 31475510

    Sample: Serum
  35. Andrologia (2018) IF: 1.588
    Testicular microanatomical and hormonal alterations following use of antiretroviral therapy in Sprague Dawley rats: Role of Naringenin

    DOI: 10.1111/and.13137

    PMID: 30168163

    Sample: Serum
  36. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  37. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  38. Plant Archives (2019)
    Sample: Serum
  39. International Journal of Basic & Clinical Pharmacology (2020)
    Ciprofloxacin and levofloxacin adversely affect male infertility indicated by pharmacological, andrological and pathological evidence

    DOI: 10.18203/2319-2003.ijbcp20200190

    Sample: Plasma
    Inhibition of leptinleptinr-kisspeptinkiss1r signaling pathway promotes follicular development in polycystic ovarian rats
    Sample: Serum
  41. NigEr?an Society for Experimental Biology (2017)
    Pregnancy and Fetal Outcomes Following Metformin Use in Diabetic Rats

    DOI: 10.1530/endoabs.50.P354

    Sample: Plasma
  42. Thai Journal of Pharmaceutical Sciences (2018)
    Apigenin and baicalin, each alone or in low-dose combination, attenuated chloroquine induced male infertility in adult rats
    Sample: Serum
  43. Pakistan Journal of Zoology (2019) IF: 0.547
    Effect of a Traditional Chinese Medicine Formula (Bu Shen Zhu Yun) on Mifepristone-Induced Abnormal Pituitary Gonadotropin Secretion in Rats.
    Sample: Serum
  44. Asian Pacific Journal of Tropical Medicine (2017) IF: 0.925
    Rescue Effects Of Aqueous Seed Extracts Of Foeniculum Vulgare And Carum Carvi Against Cadmium-Induced Hepatic, Renal And Gonadal Damage In Female Albino Rats.

    DOI: 10.1016/j.apjtm.2017.10.019

    PMID: 29268967

    Sample: Serum
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