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Rat MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

  • Cat.No.:E-EL-R0633

  • Reactivity: Rat

To Purchase E-EL-R0633

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat MCP-1 in samples. No significant cross-reactivity or interference between Rat MCP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat MCP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MCP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MCP-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.10 4.40 0.50 1.20 4.70
Standard deviation 0.03 0.05 0.15 0.03 0.07 0.17
CV (%) 6.00 4.55 3.41 6.00 5.83 3.62


The recovery of Rat MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 100
EDTA plasma (n=8) 97-111 102
Cell culture media (n=8) 86-101 93


Samples were spiked with high concentrations of Rat MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Research AreaCancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Cellular Physiology (2020) IF: 5.546
    Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators

    DOI: 10.1002/jcp.29453

    PMID: 31960959

    Sample: Cell culture supernatant,Tissue homogenate
  2. IF: 5.099
    COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism.

    DOI: 10.1016/j.envpol.2018.05.034

    PMID: 29793105

    Sample: Tissue homogenate
  3. Scientific Reports (2017) IF: 4.259
    Hydrogen Sulfide Ameliorates Chronic Renal Failure In Rats By Inhibiting Apoptosis And Inflammation Through ROS/MAPK And NF-κB Signaling Pathways.

    DOI: 10.1038/s41598-017-00557-2

    PMID: 28352125

    Sample: Tissue homogenate
  4. Molecules (2017) IF: 3.098
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  5. Phytomedicine (2018) IF: 3.61
    Epigallocatechin gallate attenuates mitochondrial DNA-induced inflammatory damage in the development of ventilator-induced lung injury.

    DOI: 10.1016/j.phymed.2018.05.017

    PMID: 30195870

    Sample: Tissue homogenate
  6. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2020) IF: 2.842
    Activation of Nrf2 Signaling by Apelin Attenuates Renal Ischemia Reperfusion Injury in Diabetic Rats

    DOI: 10.2147/DMSO.S246743

    PMID: 32606875

    Sample: Serum
  7. Bioscience reports (2019) IF: 2.942
    Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

    DOI: 10.1042/BSR20191252

    PMID: 31651935

    Sample: Serum,Plasma,Cell sample
  8. Molecules (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  9. Molecular Medicine Reports (2015) IF: 1.692
    Candesartan Ameliorates Acute Myocardial Infarction In Rats Through Inducible Nitric Oxide Synthase, Nuclear FactorκB, Monocyte Chemoattractant Protein1, Activator Protein1 And Restoration Of Heat Shock Protein 72.

    DOI: 10.3892/mmr.2015.4432

    PMID: 26499133

    Sample: Serum
  10. Journal of Experimental Pharmacology (2020)
    Alpha-Mangostin Improves Cardiac Hypertrophy and Fibrosis and Associated Biochemical Parameters in High-Fat/High-Glucose Diet and Low-Dose Streptozotocin Injection-Induced Type 2 Diabetic Rats

    DOI: 10.2147/JEP.S233111

    PMID: 32095085

    Sample: Tissue homogenate
  11. Biochem. Cell. Arch (2018)
    Sample: Serum
  12. Journal of Pharmaceutical Sciences and Research (2018)
    Role of hydroxytyrosol in ameliorating effects of high fat diet on male rats CNS
    Sample: Serum
  13. United States Patent Application Publication (2018)
    Compounds, Compositions and Methods for Prevention or Treatment of Liver and Lipid-Related Conditions
    Sample: Plasma
  14. Biochem. Cell. Arch. (2018) IF: /
    Sample: Serum
  15. bioRxiv (2018) IF: /
    Autophagy Decreases Alveolar Epithelial Cell Injury by Suppressing the NF-κB Signaling Pathway and Regulating the Release of Inflammatory Mediators.

    DOI: 10.1101/328039

    Sample: Tissue homogenate
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