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Rat MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

Cat.No.:E-EL-R0633
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat MCP-1 in samples. No significant cross-reactivity or interference between Rat MCP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat MCP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MCP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MCP-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.10	4.40	0.50	1.20	4.70
Standard deviation	0.03	0.05	0.15	0.03	0.07	0.17
CV (%)	6.00	4.55	3.41	6.00	5.83	3.62

Recovery

The recovery of Rat MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-109	100
EDTA plasma (n=8)	97-111	102
Cell culture media (n=8)	86-101	93

Linearity

Samples were spiked with high concentrations of Rat MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Research Area	Cancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Journal of Cellular Physiology (2020) IF: 5.546

Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators

DOI: 10.1002/jcp.29453
PMID: 31960959	Sample: Cell culture supernatant,Tissue homogenate

IF: 5.099

COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism.

DOI: 10.1016/j.envpol.2018.05.034
PMID: 29793105	Sample: Tissue homogenate

Scientific Reports (2017) IF: 4.259

Hydrogen Sulfide Ameliorates Chronic Renal Failure In Rats By Inhibiting Apoptosis And Inflammation Through ROS/MAPK And NF-κB Signaling Pathways.

DOI: 10.1038/s41598-017-00557-2
PMID: 28352125	Sample: Tissue homogenate

Molecules (2017) IF: 3.098

Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.

DOI: 10.3390/molecules22111956
PMID: 29137154	Sample: Cell culture supernatant

Phytomedicine (2018) IF: 3.61

Epigallocatechin gallate attenuates mitochondrial DNA-induced inflammatory damage in the development of ventilator-induced lung injury.

DOI: 10.1016/j.phymed.2018.05.017
PMID: 30195870	Sample: Tissue homogenate

Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy (2020) IF: 2.842

Activation of Nrf2 Signaling by Apelin Attenuates Renal Ischemia Reperfusion Injury in Diabetic Rats

DOI: 10.2147/DMSO.S246743
PMID: 32606875	Sample: Serum

Bioscience reports (2019) IF: 2.942

Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

DOI: 10.1042/BSR20191252
PMID: 31651935	Sample: Serum,Plasma,Cell sample

Molecules (2017) IF: 2.861

Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.

DOI: 10.3390/molecules22111956
PMID: 29137154	Sample: Cell culture supernatant

Molecular Medicine Reports (2015) IF: 1.692

Candesartan Ameliorates Acute Myocardial Infarction In Rats Through Inducible Nitric Oxide Synthase, Nuclear FactorκB, Monocyte Chemoattractant Protein1, Activator Protein1 And Restoration Of Heat Shock Protein 72.

DOI: 10.3892/mmr.2015.4432
PMID: 26499133	Sample: Serum

Journal of Experimental Pharmacology (2020)

Alpha-Mangostin Improves Cardiac Hypertrophy and Fibrosis and Associated Biochemical Parameters in High-Fat/High-Glucose Diet and Low-Dose Streptozotocin Injection-Induced Type 2 Diabetic Rats

DOI: 10.2147/JEP.S233111
PMID: 32095085	Sample: Tissue homogenate

Biochem. Cell. Arch (2018)

ASSESSMENT THE IMMUNE STIMULATING EFFECT OF PLEUROTUS OSTREATUS CRUDE EXTRACT OF MALE ALBINO RATS


	Sample: Serum

Journal of Pharmaceutical Sciences and Research (2018)

Role of hydroxytyrosol in ameliorating effects of high fat diet on male rats CNS


	Sample: Serum

United States Patent Application Publication (2018)

Compounds, Compositions and Methods for Prevention or Treatment of Liver and Lipid-Related Conditions


	Sample: Plasma

Biochem. Cell. Arch. (2018) IF: /

EVALUATE THE IMMUNE RESPONSE OF ACINETOBACTER BAUMANNII ANTIGENS IN WHITE ALBION RATS.


	Sample: Serum

bioRxiv (2018) IF: /

Autophagy Decreases Alveolar Epithelial Cell Injury by Suppressing the NF-κB Signaling Pathway and Regulating the Release of Inflammatory Mediators.

DOI: 10.1101/328039
	Sample: Tissue homogenate

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