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Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit

Cat.No.:E-EL-R0045
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat MMP-13 in samples. No significant cross-reactivity or interference between Rat MMP-13 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat MMP-13 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-13. You can calculate the concentration of Rat MMP-13 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-13 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-13 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.93	1.71	8.02	0.90	1.70	8.38
Standard deviation	0.06	0.09	0.41	0.05	0.09	0.43
CV (%)	6.45	5.26	5.11	5.56	5.29	5.13

Recovery

The recovery of Rat MMP-13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-108	102
EDTA plasma (n=8)	87-101	93
Cell culture media (n=8)	86-100	92

Linearity

Samples were spiked with high concentrations of Rat MMP-13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	MMP13, CLG3, MANDP1, Collagenase 3
Research Area	Cancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Phytotherapy Research (2017) IF: 3.092

Scopoletin-standardized Morinda Elliptica Leaf Extract Suppressed Inflammation And Cartilage Degradation To Alleviate Osteoarthritis: A preclinical Study.

DOI: 10.1002/ptr.5949
PMID: 29067744	Sample: Serum

Clinical Rheumatology (2020) IF: 2.394

Investigation of the efficacy of daidzein in experimental knee osteoarthritis-induced with monosodium iodoacetate in rats

DOI: 10.1007/s10067-020-04958-z
PMID: 32103372	Sample: Serum

Journal of Food Biochemistry (2019) IF: 1.552

Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti-inflammatory, anti-oxidative, and anti-protease pathways

DOI: 10.1111/jfbc.12755
PMID: 31353568	Sample: Serum

International Journal of Clinical and Experimental Medicine (2016) IF: 1.069

Targeting Tissue Inhibitor Of Metalloproteinase 1/2 Using A shRNA Lentiviral System Offers A Novel Treatment Strategy Against Hepatic Fibrosis.


	Sample: Serum

Experimental & Therapeutic Medicine (2015) IF: 1.261

Lentivirus-Induced Knockdown Of LRP1 Induces Osteoarthritic-Like Effects And Increases Susceptibility To Apoptosis In Chondrocytes Via The Nuclear Factor-κB Pathway.

DOI: 10.3892/etm.2015.2471
PMID: 26170918	Sample: Cell culture medium

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