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Rat NFE2L2(Nuclear Factor, Erythroid Derived 2, Like 2) ELISA Kit

Cat.No.:E-EL-R1052
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat NFE2L2 in samples. No significant cross-reactivity or interference between Rat NFE2L2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat NFE2L2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFE2L2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFE2L2. You can calculate the concentration of Rat NFE2L2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NFE2L2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NFE2L2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	48.98	139.52	386.82	51.79	143.10	368.46
Standard deviation	2.49	7.09	16.09	2.92	8.57	16.32
CV (%)	5.08	5.08	4.16	5.64	5.99	4.43

Recovery

The recovery of Rat NFE2L2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-104	96
EDTA plasma (n=8)	91-102	96
Cell culture media (n=8)	87-97	92

Linearity

Samples were spiked with high concentrations of Rat NFE2L2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Nrf2
Research Area	Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
The dual gastro- and neuroprotective effects of curcumin loaded chitosan nanoparticles against cold restraint stress in rats

DOI: 10.1016/j.biopha.2022.112778

PMID: 35272135
Sample: brain tissue
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats

DOI: 10.1016/j.ejphar.2021.174044

PMID: 33745959
Sample: Tissue homogenate(Joint Tissue)
INFLAMMOPHARMACOLOGY (2021) IF: 4.473
Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats

DOI: 10.1007/s10787-020-00785-5

PMID: 33459879
Sample: Tissue homogenate(hippocampus)
NUTRITION (2022) IF: 4.893
Acetate circumvents impaired metabolic switch in skeletal muscle of letrozole-induced PCOS rat model by suppression of PDK4/NLRP3

DOI: 10.1016/j.nut.2022.111914

Sample: skeletal muscle
NEUROSCIENCE (2023) IF: 3.708
Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate

DOI: 10.1016/j.neuroscience.2023.01.002

PMID: 36646412
Sample: cerebral cortex,cerebellum
PLoS One (2022) IF: 3.752
Short chain fatty acid, acetate restores ovarian function in experimentally induced PCOS rat model

DOI: 10.1371/journal.pone.0272124

PMID: 35881588
Sample: ovarian
MOLECULAR AND CELLULAR BIOCHEMISTRY (2023) IF: 3.842
Plantain-based diet decreases oxidative stress and inflammatory markers in the testes of rats exposed to atrazine

DOI: 10.1007/s11010-022-04639-2

Sample: Serum
INFLAMMATION (2019) IF: 2.939
Protective Effects of Tyrosol Against DSS-Induced Ulcerative Colitis in Rats

DOI: 10.1007/s10753-019-01028-8

Sample: colon tissue
Current Research in Toxicology (2022)
Zn and Se abrogate heavy metal mixture induced ovarian and thyroid oxido-inflammatory effects mediated by activation of NRF2-HMOX-1 in female albino rats

DOI: 10.1016/j.crtox.2022.100098

Sample: ovarian,thyroid,thyroid cell
Toxicology Reports (2021)
Sildenafil augments fetal weight and placental adiponectin in gestational testosterone-induced glucose intolerant rats

DOI: 10.1016/j.toxrep.2021.06.011

Sample: Placenta
Journal of Diabetes and Metabolic Disorders (2021)
Acetate supplementation restores testicular function by modulating Nrf2/PPAR-γ in high fat diet-induced obesity in Wistar rats

DOI: 10.1007/s40200-021-00924-x

Sample: testicular tissue

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