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Rat NFKB-p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

  • Cat.No.:E-EL-R0673

  • Reactivity: Rat

To Purchase E-EL-R0673

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NFKB-p105 in samples. No significant cross-reactivity or interference between Rat NFKB-p105 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p105 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p105. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p105 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p105, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFKB-p105. You can calculate the concentration of Rat NFKB-p105 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NFKB-p105 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NFKB-p105 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.47 1.42 4.64 0.51 1.36 4.23
Standard deviation 0.03 0.06 0.21 0.03 0.07 0.17
CV (%) 6.38 4.23 4.53 5.88 5.15 4.02

Recovery

The recovery of Rat NFKB-p105 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma (n=8) 92-106 98
Cell culture media (n=8) 86-99 92

Linearity

Samples were spiked with high concentrations of Rat NFKB-p105 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsNFKB1, NF-KB1, NF-Kappa-B, NFKB-p105, NFKB-p50, p105, p50, EBP-1, KBF1
Research AreaCancer, Cell Biology, Epigenetics and Nuclear Signaling, Immunology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Glutamine restores testicular glutathione-dependent antioxidant defense and upregulates NO/cGMP signaling in sleep deprivation-induced reproductive dysfunction in rats

    DOI: 10.1016/j.biopha.2022.112765

    PMID: 35247715

    Sample: Testicular,epididymal
  2. LIFE SCIENCES (2021) IF: 5.037
    Protective effects of hesperidin through attenuation of Ki67 expression against DMBA-induced breast cancer in female rats

    DOI: 10.1016/j.lfs.2021.119957

    PMID: 34530017

    Sample: Breast tissue
  3. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 5.572
    Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway

    DOI: 10.1016/j.fct.2022.113471

    PMID: 36270409

    Sample: liver,kidney
  4. BRAIN RESEARCH BULLETIN (2021) IF: 4.079
    Effect of lobeglitazone on motor function in rat model of Parkinson’s disease with diabetes co-morbidity

    DOI: 10.1016/j.brainresbull.2021.05.011

    Sample: Tissue homogenate
  5. Journal of Traditional and Complementary Medicine (2021) IF: 4.221
    Menhaden fish oil attenuates postpartum depression in rat model via inhibition of NLRP3-inflammasome driven inflammatory pathway

    DOI: 10.1016/j.jtcme.2021.02.007

    Sample: brain
  6. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats

    DOI: 10.1007/s10787-020-00785-5

    PMID: 33459879

    Sample: Tissue homogenate(hippocampus)
  7. TOXICOLOGY (2022) IF: 4.571
    Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF‑kB signaling in female albino rats

    DOI: 10.1016/j.tox.2022.153350

    PMID: 36220500

    Sample: liver,kidney
  8. NEUROSCIENCE (2023) IF: 3.708
    Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate

    DOI: 10.1016/j.neuroscience.2023.01.002

    PMID: 36646412

    Sample: cerebral cortex,cerebellum
  9. ENDOCRINE (2022) IF: 3.925
    Acetate restores hypothalamic-adipose kisspeptin status in a rat model of PCOS by suppression of NLRP3 immunoreactivity

    DOI: 10.1007/s12020-022-03191-9

    PMID: 36114434

    Sample: hypothalamic tissue,VAT
  10. NEUROCHEMICAL RESEARCH (2022) IF: 3.996
    Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats

    DOI: 10.1007/s11064-022-03640-x

    PMID: 35665448

    Sample: hippocampal tissue
  11. Metabolic Syndrome and Related Disorders (2022) IF: 2.363
    Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine

    DOI: 10.1089/met.2022.0028

    PMID: 35796694

    Sample: Liver tissue
  12. PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
    Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells

    DOI: 10.1080/13880209.2020.1772840

    Sample: Cell culture supernatant
  13. Pharmacognosy Magazine (2017) IF: 1.069
    The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats

    DOI: 10.4103/pm.pm_134_17

    Sample: serum
  14. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    Sample: liver
  15. Journal of Diabetes and Metabolic Disorders (2023)
    Effect of Oleanolic acid administration on hepatic AMPK, SIRT-1, IL-6 and NF-κB levels in experimental diabetes

    DOI: 10.1007/s40200-022-01178-x

    Sample: Liver tissue
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