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Rat PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

  • Cat.No.:E-EL-R0792

  • Reactivity: Rat

To Purchase E-EL-R0792

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 125.00-8000 pg/mL
Sensitivity 75.00 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Rat PTGS2/COX-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTGS2/COX-2. You can calculate the concentration of Rat PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PTGS2/COX-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PTGS2/COX-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 403.31 795.28 3388.82 400.56 815.30 3160.09
Standard deviation 27.43 46.92 102.34 26.44 41.17 165.59
CV (%) 6.80 5.90 3.02 6.60 5.05 5.24


The recovery of Rat PTGS2/COX-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-96 90
EDTA plasma (n=8) 90-103 97
Cell culture media (n=8) 91-103 97


Samples were spiked with high concentrations of Rat PTGS2/COX-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCOX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Research AreaCancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. International Journal of Biological Macromolecules (2020) IF: 5.162
    Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management

    DOI: 10.1016/j.ijbiomac.2020.07.133

    PMID: 32693125

    Sample: Serum
  2. Lasers in Surgery and Medicine (2019) IF: 3.02
    Effects of Pulsed 810 nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

    DOI: 10.1002/lsm.23156

    PMID: 31483061

    Sample: Tissue homogenate
  3. Molecules (2017) IF: 3.098
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  4. Acta Cirurgica Brasileira (2018) IF: 3.0895
    Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation.

    DOI: 10.1590/s0102-865020180030000006

    PMID: 29668774

    Sample: Serum
  5. Journal of Biochemical And Molecular Toxicology (2017) IF: 2.042
    Protective Effect Of Proanthocyanidins Against Doxorubicin-induced Nephrotoxicity In Rats.

    DOI: 10.1002/jbt.21965

    PMID: 28759702

    Sample: Tissue homogenate
  6. Molecules (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  7. Journal of Biochemical and Molecular Toxicology (2017) IF: 2.042
    Alpha Lipoic Acid Prevents Doxorubicin-Induced Nephrotoxicity By Mitigation Of Oxidative Stress, Inflammation, And Apoptosis In Rats.

    DOI: 10.1002/jbt.21940

    PMID: 28598563

    Sample: Tissue homogenate
  8. Molecules (2016) IF: 2.861
    Zerumbone, A Bioactive Sesquiterpene, Ameliorates Diabetes-Induced Retinal Microvascular Damage Through Inhibition Of Phospho-P38 Mitogen-Activated Protein Kinase And Nuclear Factor-κB Pathways.

    DOI: 10.3390/molecules21121708

    PMID: 27973425

    Sample: Tissue homogenate
  9. Naunyn-Schmiedeberg's archives of pharmacology (2020)
    Protective effect of oleuropein on ketamine-induced cardiotoxicity in rats

    DOI: 10.1007/s00210-020-01870-w

    PMID: 32383030

    Sample: Tissue homogenate
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