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Rat TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

  • Cat.No.:E-EL-R0939

  • Reactivity: Rat

To Purchase E-EL-R0939

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.63-40 ng/mL
Sensitivity 0.38 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat TRACP-5b in samples. No significant cross-reactivity or interference between Rat TRACP-5b and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat TRACP-5b concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TRACP-5b. You can calculate the concentration of Rat TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TRACP-5b were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TRACP-5b were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.07 5.59 19.86 2.26 5.89 20.98
Standard deviation 0.12 0.24 0.68 0.15 0.24 0.72
CV (%) 5.80 4.29 3.42 6.64 4.07 3.43


The recovery of Rat TRACP-5b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-98 93
EDTA plasma (n=8) 93-109 99
Cell culture media (n=8) 84-98 90


Samples were spiked with high concentrations of Rat TRACP-5b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


    Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats

    DOI: 10.1186/s12951-021-00976-4

    PMID: 34407835

    Sample: serum
  2. LIFE SCIENCES (2022) IF: 5.037
    Hyperbaric oxygen therapy improves age induced bone dyshomeostasis in non-obese and obese conditions

    DOI: 10.1016/j.lfs.2022.120406

    PMID: 35182555

    Sample: serum
  3. Frontiers in Endocrinology (2021) IF: 5.555
    Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

    DOI: 10.3389/fendo.2021.759843

    PMID: 34777254

    Sample: serum
    Cycloastragenol prevents age-related bone loss: Evidence in d-galactose-treated and aged rats

    DOI: 10.1016/j.biopha.2020.110304

    PMID: 32497865

    Sample: Serum
    The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

    DOI: 10.3390/ijms21041526

    Sample: blood
    Osteoprotective effects of estrogen membrane receptor GPR30 in ovariectomized rats

    DOI: 10.1016/j.jsbmb.2015.07.002

    Sample: Serum
  7. ENDOCRINE (2022) IF: 3.925
    Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling

    DOI: 10.1007/s12020-022-03199-1

    PMID: 36184719

    Sample: serum
  8. Acupuncture in Medicine (2018) IF: 2.275
    Long-Term Electroacupuncture Stimulation Prevents Osteoporosis in Ovariectomised Osteopaenic Rats through Multiple Signalling Pathways:

    DOI: 10.1136/acupmed-2016-011268

    Sample: serum
  9. Journal of Orthopaedic Surgery and Research (2022) IF: 2.359
    Roxadustat promotes osteoblast differentiation and prevents estrogen deficiency-induced bone loss by stabilizing HIF-1α and activating the Wnt/β-catenin signaling pathway

    DOI: 10.1186/s13018-022-03162-w

    PMID: 35597989

    Sample: serum
  10. BIOSCIENCE REPORTS (2015) IF: 2.637
    Osteoprotective effect of combination therapy of low-dose oestradiol with G15, a specific antagonist of GPR30/GPER in ovariectomy-induced osteoporotic rats

    DOI: 10.1042/BSR20150146

    Sample: Serum
  11. Experimental and Therapeutic Medicine (2015) IF: 1.269
    Electroacupuncture at the governor vessel and bladder meridian acupoints improves postmenopausal osteoporosis through osteoprotegerin/RANKL/RANK and Wnt/β‑catenin signaling pathways

    DOI: 10.3892/etm.2015.2553

    Sample: Serum
  12. Advances in Traditional Medicine (2019)
    Ameliorative potential of Saudi Arabian date fruit (Phoenix dactylifera L.) varieties against Freund’s complete adjuvant induced arthritis in rats

    DOI: 10.1007/s13596-019-00377-x

    Sample: serum
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