RB1 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC-p
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Verified Samples |
Verified Samples in WB:3T3 |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:300 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Synthesized peptide derived from human Rb around the non-phosphorylation site of S807. |
Abbre | Rb |
Synonyms | Exon 17 tumor GOS561 substitution mutation causes premature stop;GOS563 exon 17 substitution mutation causes premature stop;OSRC;Osteosarcoma;p105-Rb;P105RB;PP105;pp110;PPP1R130;pRb;Prepro retinoblastoma associated protein;Protein phosphatase 1 regulatory subunit 130;Rb;RB transcriptional corepressor 1;RB;RB1;RB1 gene;Retinoblastoma 1;Retinoblastoma suspectibility protein;Retinoblastoma-associated protein |
Swissprot | |
Calculated MW | 106kDa |
Observed MW |
106kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide,0.5% protective protein and 50% glycerol pH 7.4. |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. |
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Coordinated regulation of cell survival and cell cycle pathways by DDR2-dependent SRF transcription factor in cardiac fibroblasts
IF:3.864
Journal:AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
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The Molecular Chaperone HSP70 Controls Liver Cancer Initiation and Progression by Regulating Adaptive DNA-Damage and MAPK/ERK Signaling Pathways
IF:3.735
Journal:MOLECULAR AND CELLULAR BIOLOGY
PMID:30745413