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RBM15 Polyclonal Antibody

Cat:E-AB-67632
Manual MSDS

Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:HL-60,HepG2
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human RBM15 (NP_001188474.1).
Abbre RBM15
Synonyms RBM15;OTT;OTT1;SPEN
Swissprot
Calculated MW 99kDa/104kDa/106kDa/107kDa
Observed MW 120kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 1mg/mL
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Epigenetics and Nuclear Signaling; Stem Cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family,which have repressor function in several signaling pathway,and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia,and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation,which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation,block the cell cycle,and induce apoptosis in CML cells.