Reactive Oxygen Species (ROS) Fluorometric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K138-F

      Size:
      • 96T
      Qty:
      - +
      Price: $280
      Can detect 96 samples if without duplication

      Detection method: Fluorescence method

      Detection instrument: Fluorescence Microplate reader (Ex/Em=500 nm/525 nm), Fluorescence Spectrophotometer (Ex/Em=500 nm/5

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

      Performance characteristics

      Synonyms ROS
      Sample type Fresh tissue,cultured cells
      Detection method Fluorescence method
      Assay type Cell-based (quantitative)
      Assay time 60 min
      Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
      Storage Reagent 1: -20℃, Reagent 2: 2-8℃
      Valid period 6 months

      Citations

      1. Exendin-4 Attenuates Remodeling in the Remote Myocardium of Rats After an Acute Myocardial Infarction by Activating β-Arrestin-2, Protein Phosphatase 2A, and Glycogen Synthase Kinase-3 and Inhibiting β-Catenin
        IF: 4.069
        Journal: Cardiovascular Drugs and Therapy(2020)
        DOI: 10.1007/s10557-020-07006-9
        PMID: 32474680
        Products: E-BC-K138-F 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. STUDY OF PURIFIED MICROCYSTIN-LR TOXICITY ON BIOCHEMICAL MARKERS IN BLOOD AND LIVER OF CYPRINUS CARPIO DURING ACUTE EXPOSURE
        IF: 0.1
        Journal: Plant Archives(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K138-F 
        Sample type: Tissue homogenate
        Reactivity: Fish
      3. Evaluating the Bystander Effects of Electron Beam Irradiation in Radiotherapy on MCF-7 Human Breast Cancer Cells
        Journal: Preprint(2019)
        DOI: 10.21203/rs.2.17986/v1
        PMID: 99999999
        Products: E-BC-K138-F 
        Sample type: Cell sample
        Reactivity: Human
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      • Q&A (1)
      Q

      a********dSubmitted [ Apr 06 2021 ]

      Asked: What is the procedure for this assay?

      Reply

      A

      adminSubmitted [ Apr 06 2021 ]

      Answered: Hi, Thanks for your interests in our products. There are detailed operation in the manual. Can we know where didn't we make clear? If you need any help, pls feel free to ask. Best regards, Hanny

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