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Recombinant 2019-nCoV NSP7 Protein (His Tag)

Uniprot : YP_009725303.1
  • Cat.No.:PKSR030469

  • Expression host: E.coli

To Purchase PKSR030469

Size:
  • 10µg
  • 50µg
Price: $220
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Description

Synonyms SARS-CoV 2 nsp7
Species Virus
Expression_host E.coli
Sequence Ser1-Gln83
Accession YP_009725303.1
Mol_Mass 12.3 kDa
AP_Mol_Mass 12 kDa
Tag C-6His

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Formulation Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
Reconstitution Please refer to the printed manual for detailed information.

Background

The ∼30kb positive-stranded RNA genome of coronaviruses encodes a replication/transcription machinery that is unusually complex and composed of 16 nonstructural proteins (nsps). The four proteins nsp7 to nsp10, which are conserved among all CoVs but have no functional homologs outside of the Coronaviridae, are translated as part of the viral polyproteins pp1a and pp1ab, and the mature proteins are released by the action of the SARS-CoV protease nsp5. Hexadecamer of nsp7 and nsp8 may possess dsRNA-binding activity. 2019-nCoV nonstructural protein 7 (nsp7) is of interest for its potential roles in the transcription and replication of the positive-stranded viral RNA genome.

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